HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte
HL-1 cells: A cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte
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DOI：
10.1073/pnas.95.6.2979
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发表时间：
1998-03-17
影响因子：
11.1
通讯作者：
Izzo, NJ
中科院分区：
文献类型：
--
作者：
Claycomb, WC;Lanson, NA;Izzo, NJ
我们从AT-1小鼠心房心肌细胞肿瘤谱系中获得了一个心肌细胞系，命名为HL-1。H-1细胞可连续传代，但仍能保持收缩能力，并保持分化的心脏形态、生化和电生理特性。在培养的HL-1细胞中发现了胚胎心房肌细胞的典型超微结构特征。基于逆转录酶-PCR的分析证实了与成人心房肌细胞相似的基因表达模式，包括α-心肌肌球蛋白重链、α-心肌肌动蛋白和连接蛋白43的表达，它们还表达心房利钠因子的基因。HL-1细胞的免疫组织化学染色表明，心脏特异性标志物结蛋白、肌节肌球蛋白和心房利钠因子的分布与培养的心房心肌细胞的分布相似。HL-1细胞外向电流以延迟整流钾电流（I-Kr）为主，激活电流呈内向整流，失活电流呈电压依赖性，在>> + 20 mV时达到饱和，对多非利特高度敏感（IC_（50）为46.9 nM）。[H-3]多非利特的特异性结合是可饱和的，符合单点结合等温线，Kd为140 +/-60 nM，B-max为118 fmol/10（5）个细胞。HL-1细胞代表了一种心肌细胞系，可以重复传代，但仍保持心脏特异性表型。
We have derived a cardiac muscle cell line, designated HL-1, from the AT-1 mouse atrial cardiomyocyte tumor lineage. H-1 tells can be serially passaged, yet they maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties, Ultrastructural characteristics typical of embryonic atrial cardiac muscle cells were found consistently in the cultured HL-1 cells, Reverse transcriptase-PCR-based analyses confirmed a pattern of gene expression similar to that of adult atrial myocytes, including expression of alpha-cardiac myosin heavy chain, alpha-cardiac actin, and connexin43, They also express the gene for atrial natriuretic factor, Immunohistochemical staining of the HL-1 tells indicated that the distribution of the cardiac-specific markers desmin, sarcomeric myosin, and atrial natriuretic factor was similar to that of cultured atrial cardiomyocytes. A delayed rectifier potassium current (I-Kr) was the most prominent outward current in HL-1 cells, The activating currents displayed inward rectification and deactivating current tails were voltage-dependent, saturated at >> + 20 mV, and were highly sensitive to dofetilide (IC50 of 46.9 nM). Specific binding of [H-3]dofetilide was saturable and fit a one-site binding isotherm with a K-d of 140 +/- 60 nM and a B-max of 118 fmol per 10(5) cells, HL-1 cells represent a cardiac myocyte cell line that can be repeatedly passaged and yet maintain a cardiac-specific phenotype.