The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila.
The Mcp element mediates stable long-range chromosome-chromosome interactions in Drosophila.
复制标题
DOI：
10.1091/mbc.e06-01-0049
复制
发表时间：
2006-05
影响因子：
3.3
通讯作者：
J. Vazquez;Martin Müller;V. Pirrotta;J. Sedat
中科院分区：
文献类型：
--
作者：
J. Vazquez;Martin Müller;V. Pirrotta;J. Sedat
细胞核内的染色体组织不是随机的，而是由多种因素决定的，包括染色体与核成分（如核膜或核基质）之间的相互作用。这种相互作用可能对正确的核组织、细胞分裂过程中的染色体分裂和基因调控至关重要。一个重要的，但很少记录的子集，包括特定染色体区域之间的相互作用。这种类型的相互作用被认为是参与远程启动子调控的远程增强子或基因座控制区域，并可能是横切等现象的基础。在这里，我们使用了一种基于Lac阻遏因子/操作符识别的体内显微镜分析，表明来自果蝇双胸复合体的多梳响应元件Mcp能够介导远程染色体区域之间的物理相互作用。这些相互作用是组织特异性的，可以在多个Mcp元素之间发生，并且一旦建立似乎是稳定的。我们推测这种相互作用的能力可能是Mcp在双胸复合体中调节其调节功能的机制的一部分。
Chromosome organization inside the nucleus is not random but rather is determined by a variety of factors, including interactions between chromosomes and nuclear components such as the nuclear envelope or nuclear matrix. Such interactions may be critical for proper nuclear organization, chromosome partitioning during cell division, and gene regulation. An important, but poorly documented subset, includes interactions between specific chromosomal regions. Interactions of this type are thought to be involved in long-range promoter regulation by distant enhancers or locus control regions and may underlie phenomena such as transvection. Here, we used an in vivo microscopy assay based on Lac Repressor/operator recognition to show that Mcp, a polycomb response element from the Drosophila bithorax complex, is able to mediate physical interaction between remote chromosomal regions. These interactions are tissue specific, can take place between multiple Mcp elements, and seem to be stable once established. We speculate that this ability to interact may be part of the mechanism through which Mcp mediates its regulatory function in the bithorax complex.