Solanum lycopersicon Mill. and Nicotiana benthamiana L. under high light show distinct responses to anti-oxidative stress
Solanum lycopersicon Mill. and Nicotiana benthamiana L. under high light show distinct responses to anti-oxidative stress
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DOI：
10.1016/j.jplph.2007.04.009
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Amancio, Sara
中科院分区：
文献类型：
--
作者：
Carvalho, Luisa C.;Santos, Sonia;Amancio, Sara
两个实验上重要的物种，茄lycopersicon米尔。和本氏烟草（Nicotiana benthamiana L.），在低密度（50 μ mol m（-2）s（-1））下进行体外繁殖，并根据先前为葡萄藤和栗子开发的方案转移到HL（200 μ mol m（-2）s（-1））。与已经在这些物种中测试的光氧化胁迫参数相比，过氧化氢和超氧化物的成像显示在d2-3和d 6在S.番茄的d1-2和d5-7;本萨米亚纳SOD、CAT和APX活性与ROS积累相匹配。在S.而番茄属N.本塞姆氏菌属（BenthamianaL.）发现了双峰模式，在D2和D 7具有峰。这些结果，再加上根扩展和新叶出现的相对时间，表明这两个明显相似的物种显示不同的策略时，响应光胁迫，进一步证明了每个物种的反应的独特性。研究了N. Benthamiana福尔斯与已经报道的包括葡萄藤的木材种类的模式非常接近。(c)2007年，Elsevier GmbH。All rights reserved.
Two experimentally important species, Solanum lycopersicon Mill. and Nicotiana benthamiana L., were propagated in vitro under low tight (50 mu mol m(-2) s(-1)) and transferred to HL (200 mu mol m(-2) s(-1)) under a protocol previously developed for grapevine and chestnut. Compared with photooxidative stress parameters already tested in those species, imaging of hydrogen peroxide and superoxide revealed an accumulation on d2-3 and d6 in S. lycopersicon and d1-2 and d5-7 in N. benthamiana. SOD, CAT and APX activities matched ROS accumulation. The expression of the respective transcripts showed a significant increase on d1 in S. lycopersicon while in N. benthamiana a bimodal pattern was found, with peaks on d2 and d7. These results, together with the relative timing of root expansion and new leaf emergence, indicate that these two apparently similar species display different strategies when responding to light stress, evidencing further the uniqueness of the response of each species. The behaviour of N. benthamiana falls closely into the pattern already reported for wood species including grapevine. (c) 2007 Elsevier GmbH. All rights reserved.