Fabrication and characterization of Mg-doped chitosan-gelatin nanocompound coatings for titanium surface functionalization
Fabrication and characterization of Mg-doped chitosan-gelatin nanocompound coatings for titanium surface functionalization
复制标题
用于钛表面功能化的镁掺杂壳聚糖-明胶纳米复合涂层的制备和表征
DOI：
10.1080/09205063.2016.1170416
复制
发表时间：
2016-01-01
影响因子：
3.6
通讯作者：
Wang, Yining
中科院分区：
文献类型：
--
作者：
Cai, Xinjie;Cai, Jing;Wang, Yining
钛及其合金已广泛应用于临床并取得巨大成功。由于钛表面的生物惰性，在一些妥协的条件下仍然存在挑战。本研究旨在通过电泳沉积（EPD）使用镁（Mg）掺杂的壳聚糖/明胶（CS/G）纳米化合物涂层对钛表面进行功能化。通过EPD在钛基体上成功制备了负载不同镁量的CS/G涂层。涂层的物理化学表征证实镁离子以剂量依赖性方式加载到涂层中。 XRD结果表明，镁的共沉积影响了涂层的结晶度，并出现了一种新的结晶物质，即水合碱式碳酸镁。机械测试表明，镁掺杂涂层的拉伸和剪切结合强度得到改善，而过高的镁浓度最终会降低结合强度。 ICP-OES 在 28 天内检测到镁离子的持续释放。 TEM 图像还显示纳米颗粒可以从涂层中释放出来。体外细胞反应测定表明，与 CS/G 涂层相比，掺镁纳米化合物涂层可以增强 MC3T3-E1 细胞的增殖和成骨分化。因此，可以得出结论，通过EPD在钛基体上成功制备了Mg掺杂CS/G纳米化合物涂层。用这种有机-无机纳米化合物涂层对钛表面进行功能化将是一个有前途的候选者。
Titanium and its alloys have been widely used in clinic and achieved great success. Due to the bio-inertness of titanium surface, challenges still exit in some compromised conditions. The present study aimed to functionalize titanium surface with magnesium (Mg)-doped chitosan/gelatin (CS/G) nanocompound coatings via electrophoretic deposition (EPD). CS/G coatings loaded with different amount of magnesium were successfully prepared on titanium substrate via EPD. Physicochemical characterization of the coatings confirmed that magnesium ions were loaded into the coatings in a dose-dependent manner. XRD results demonstrated that co-deposition of magnesium influenced the crystallinity of the coatings, and a new crystalline substance presented, namely hydrated basic magnesium carbonate. Mechanical tests showed improved tensile and shear bond strength of the magnesium-doped coatings, while the excessively high magnesium concentration could eventually decrease the bonding strength. Sustained release of magnesium ion was detected by ICP-OES within 28 days. TEM images also displayed that nanoparticles could be released from the coatings. In vitro cellular response assays demonstrated that the Mg-doped nanocompound coatings could enhance the proliferation and osteogenic differentiation of MC3T3-E1 cells compared to CS/G coatings. Therefore, it could be concluded that Mg-doped CS/G nanocompound coatings were successfully fabricated on titanium substrates via EPD. It would be a promising candidate to functionalize titanium surface with such organic-inorganic nanocompound coatings.