Differential expression of PE and PE_PGRS genes in Mycobacterium tuberculosis strains
Differential expression of PE and PE_PGRS genes in Mycobacterium tuberculosis strains
复制标题
DOI：
10.1016/s0378-1119(03)00751-0
复制
发表时间：
2003-10-30
期刊：
影响因子：
3.5
通讯作者：
Espitia, C
中科院分区：
文献类型：
--
作者：
Flores, J;Espitia, C
本研究采用逆转录聚合酶链反应（RT-PCR）技术，检测了结核分枝杆菌PE，PE_polimorphic GC-rich sequences（PGRS）基因家族编码约99种富含甘氨酸蛋白的基因在结核分枝杆菌中的表达。结核分枝杆菌H37 Rv、卡氏分枝杆菌（Mycobacterium canettii）和2株临床分离的结核分枝杆菌（M.结核RT-PCR产物的限制性内切酶分析和测序结果表明，所有菌株均表达PE Rv 1172 c基因，而PE_PGRS Rv 3652基因仅在一株M. PE_PGRS Rv 0578 c在结核分枝杆菌临床分离株中不表达。卡内蒂。还确定了PE_PGRS Rv 0278 c和Rv 0279 c不被任何研究的菌株表达。本报告中提供的数据表明，PE、PE_PGRS基因在M.结核菌株在体外生长过程中。这些结果表明，PE、PE_PGRS基因可能在M.结核菌株(C)2003 Elsevier B.V保留所有权利。
In this study, the expression of the Mycobacterium tuberculosis PE, PE_polimorphic GC-rich sequences (PGRS) gene family encoding approximately 99 glycine-rich proteins was assayed by reverse-transcriptase polymerase chain reaction (RT-PCR) in M. tuberculosis H37Rv, Mycobacterium canettii and two clinical isolates of M. tuberculosis. Restriction analyses and sequencing of the RT-PCR products showed that all the strains expressed the PE Rv1172c gene while the PE_PGRS Rv3652 gene was only expressed by one of the M. tuberculosis clinical isolates, and the PE_PGRS Rv0578c was not expressed by M. canettii. It was also determined that the PE_PGRS Rv0278c and Rv0279c were not expressed by any of the studied strains. The data presented in this report show that the PE, PE_PGRS genes are differentially expressed in M. tuberculosis strains during in vitro growth. These findings suggest that PE, PE_PGRS genes may play a role in protein variation between M. tuberculosis strains. (C) 2003 Elsevier B.V All rights reserved.