Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis.
Multiple conserved regulatory elements with overlapping functions determine Sox10 expression in mouse embryogenesis.
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DOI：
10.1093/nar/gkm727
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发表时间：
2007
影响因子：
14.9
通讯作者：
Wegner M
中科院分区：
文献类型：
--
作者：
Werner T;Hammer A;Wahlbuhl M;Bösl MR;Wegner M
转录因子Sox 10的表达和功能在神经嵴细胞、其衍生物和少突胶质细胞中占主导地位。为了了解Sox 10表达在发育过程中是如何调节的，我们分析了Sox 10基因组区域中进化保守的非编码序列作为增强子的潜力。通过将这些序列连接到在最小启动子控制下的β-半乳糖苷酶标记基因，鉴定了五个调节区，其以限定的时间模式将转基因小鼠中的标记基因表达引导至表达Sox 10的细胞类型和组织。这些可能的Sox 10基因的增强子介导Sox 10在耳泡、少突胶质细胞和几种神经嵴衍生物（包括发育中的外周神经系统和肾上腺）中的表达。它们还表现出重叠的活性，并共享Sox、Lef/Tcf、Pax和AP 2转录因子的结合位点。这可能解释了Sox 10在胚胎发育过程中的高水平和稳健性表达。
Expression and function of the transcription factor Sox10 is predominant in neural crest cells, its derivatives and in oligodendrocytes. To understand how Sox10 expression is regulated during development, we analysed the potential of evolutionary conserved non-coding sequences in the Sox10 genomic region to function as enhancers. By linking these sequences to a β-galactosidase marker gene under the control of a minimal promoter, five regulatory regions were identified that direct marker gene expression in transgenic mice to Sox10 expressing cell types and tissues in a defined temporal pattern. These possible enhancers of the Sox10 gene mediate Sox10 expression in the otic vesicle, in oligodendrocytes and in several neural crest derivatives including the developing peripheral nervous system and the adrenal gland. They furthermore exhibit overlapping activities and share binding sites for Sox, Lef/Tcf, Pax and AP2 transcription factors. This may explain high level and robustness of Sox10 expression during embryonic development.
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影响因子：
5.3
作者：
Kuhlbrodt, K;Herbarth, B;Wegner, M
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