HEPATIC-UPTAKE OF BILE-ACIDS IN MAN - FASTING AND POSTPRANDIAL CONCENTRATIONS OF INDIVIDUAL BILE-ACIDS IN PORTAL VENOUS AND SYSTEMIC BLOOD-SERUM
HEPATIC-UPTAKE OF BILE-ACIDS IN MAN - FASTING AND POSTPRANDIAL CONCENTRATIONS OF INDIVIDUAL BILE-ACIDS IN PORTAL VENOUS AND SYSTEMIC BLOOD-SERUM
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DOI：
10.1172/jci110668
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发表时间：
1982-01-01
影响因子：
15.9
通讯作者：
EWERTH, S
中科院分区：
文献类型：
--
作者：
ANGELIN, B;BJORKHEM, I;EWERTH, S
进行了一项研究，以表征餐后胆汁酸流入肝脏的情况，并确定外周静脉胆汁酸水平是否始终充分反映门静脉浓度，或者在生理条件下是否会发生肝胆汁酸摄取饱和。 5例无并发症的胆固醇胆石病患者，在胆囊切除术时进行脐带插管，并在左门支留置导管5-7天。然后在标准化膳食前后每隔 15-30 分钟测定门静脉和体循环中胆酸、鹅去氧胆酸和脱氧胆酸的血清浓度。使用了高度准确且特异的气相色谱/质谱技术。 3 种胆汁酸的空腹浓度之和平均为 14.04.+-。门静脉血清中为4.13.mu.mol/l，以及2.44.+-。外周静脉血清中0.31μmol/l。估计的胆酸肝脏摄取分数为.apprx。 90%，鹅去氧胆酸和脱氧胆酸为70-80%。这导致二羟基胆汁酸种类中全身胆汁酸的富集。标准化膳食后，门静脉胆汁酸浓度增加 2 至 6 倍，餐后 15-60 分钟达到峰值。餐后门静脉最大胆汁酸浓度平均为43.04±。 6.12μmol/l，外周血清中相应浓度为5.22±。 0.74μmol/升。单个胆汁酸的估计吸收分数不受肝脏流入量增加的影响。个体的外周静脉浓度以及总胆汁酸与门静脉血清中的浓度有很好的相关性。结果对流入肝脏的餐后胆汁酸进行了定量，并表明健康人的肝脏胆汁酸摄取系统在内源性胆汁酸最大流入期间不能饱和。因此，外周血清胆汁酸的测量可以提供有关肠肝循环状态的重要信息。
An investigation was undertaken to characterize the postprandial inflow of individual bile acids to the liver and determine if peripheral venous bile acid levels always adequately reflect the portal venous concentration, or if saturation of hepatic bile acid uptake can occur under physiological conditions. In 5 patients with uncomplicated cholesterol gallstone disease, the umbilical cord was cannulated during cholecystectomy, and a catheter was left in the left portal branch for 5-7 days. The serum concentrations of cholic acid, chenodeoxycholic acid, and deoxycholic acid in portal venous and systemic circulation were then determined at intervals of 15-30 min before and after a standardized meal. A highly accurate and specific gas chromatographic/mass spectrometric technique was used. The sum of the fasting concentrations of the 3 bile acids averaged 14.04 .+-. 4.13 .mu.mol/l in portal venous serum, and 2.44 .+-. 0.31 .mu.mol/l in peripheral venous serum. The estimated hepatic fractional uptake of cholic acid was .apprx. 90%, and those of chenodeoxycholic acid and deoxycholic acid were 70-80%. This resulted in an enrichment of systemic bile acids in the dihydroxy bile acid species. In response to a standardized meal, portal venous bile acid concentrations increased 2- to 6-fold, with a peak seen 15-60 min after the meal. The maximum postprandial portal venous bile acid concentration averaged 43.04 .+-. 6.12 .mu.mol/l, and the corresponding concentration in peripheral serum was 5.22 .+-. 0.74 .mu.mol/l. The estimated fractional uptakes of the individual bile acids were not affected by the increased inflow to the liver. The peripheral venous concentrations of individual as well as total bile acids were well correlated with those in portal venous serum. The results give a quantitation of postprandial bile acid inflow to the liver and indicate that the hepatic uptake system for bile acids in health man cannot be saturated during maximal inflow of endogenous bile acids. Measurement of peripheral serum bile acids can thus give important information on the status of the enterohepatic circulation.