Positive MRI contrast enhancement in THP-1 cells with Gd2O3 nanoparticles
Positive MRI contrast enhancement in THP-1 cells with Gd2O3 nanoparticles
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DOI：
10.1002/cmmi.236
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发表时间：
2008-05-01
影响因子：
--
通讯作者：
Engstrom, Maria
中科院分区：
文献类型：
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作者：
Klasson, Anna;Ahren, Maria;Engstrom, Maria
人们需要更有效和组织特异性的 MRI 造影剂，最近的发展涉及可用作分子标记和磁性示踪剂的物质的设计。在这项研究中，研究了氧化钆 (Gd2O3) 纳米粒子的细胞标记和产生正对比的能力。使用 THP-1（一种具有吞噬能力的单核细胞系）并将结果与细胞培养基 (RPMI 1640) 中颗粒的弛豫度进行比较。结果显示，与未处理的细胞相比，Gd2O3 标记的细胞具有更短的 T-1 和 T-2 弛豫时间。观察到信号强度的显着差异，表明 Gd2O3 纳米粒子可以用作细胞标记的阳性造影剂。细胞样品的r(1)为4.1，细胞培养基的r(1)为3.6s(-1)mm(-1)。 r(2)分别为17.4和12.9s(-1)mm(-1)。对于 r(1)，与细胞培养基中的颗粒相比，细胞中颗粒之间的弛豫率没有显着差异 (p(r1) = 0.36)，但两个不同系列的 r(2) 显着不同 (p(r2) = 0.02)。活力结果表明，THP-1 细胞可以长时间忍受 Gd2O3 纳米粒子的处理，因此得出结论，本研究的结果基于活细胞。版权所有 (c) 2008 John Wiley & Sons, Ltd.
There is a demand for more efficient and tissue-specific MRI contrast agents and recent developments involve the design of substances useful as molecular markers and magnetic tracers. In this study, nanoparticles of gadolinium oxide (Gd2O3) have been investigated for cell labeling and capacity to generate a positive contrast. THP-1, a monocytic cell line that is phagocytic, was used and results were compared with relaxivity of particles in cell culture medium (RPMI 1640). The results showed that Gd2O3-labeled cells have shorter T-1 and T-2 relaxation times compared with untreated cells. A prominent difference in signal intensity was observed, indicating that Gd2O3 nanoparticles can be used as a positive contrast agent for cell labeling. The r(1) for cell samples was 4.1 and 3.6s(-1) mm(-1) for cell culture medium. The r(2) was 17.4 and 12.9s(-1) mm(-1), respectively. For r(1), there was no significant difference in relaxivity between particles in cells compared to particles in cell culture medium, (p(r1) = 0.36), but r(2) was significantly different for the two different series (p(r2) = 0.02). Viability results indicate that THP-1 cells endure treatment with Gd2O3 nanoparticles for an extended period of time and it is therefore concluded that results in this study are based on viable cells. Copyright (c) 2008 John Wiley & Sons, Ltd.