Role of membrane charge and semiquinone structure on naphthosemiquinone derivatives and 1,4-benzosemiquinone disproportionation and membrane-buffer distribution coefficients.
Role of membrane charge and semiquinone structure on naphthosemiquinone derivatives and 1,4-benzosemiquinone disproportionation and membrane-buffer distribution coefficients.
复制标题
膜电荷和半醌结构对萘半醌衍生物和 1,4-苯并半醌歧化和膜缓冲液分配系数的作用。
DOI：
10.1080/10715760100301541
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发表时间：
2001
影响因子：
3.3
通讯作者：
Cordones,E
中科院分区：
文献类型：
--
作者：
Alegría,AE;Santiago,G;Lópes,M;Rosario,BI;Cordones,E
本文测定了1，2-萘醌（ONQ·-）、1，4-萘醌（NQ·-）及其两种羟基化衍生物5，8-二羟基-1，4-萘醌（NZQ·-）和5-羟基-1，4-萘醌（NZQ·-）的半醌膜/缓冲液分配系数。4-萘醌（JQ·-）作为磷脂酰胆碱（PC）多层囊泡膜电荷的函数以及该脂质和磷脂酸（PC：PA）和十六烷基三甲基溴化铵（PC：CTAB）在生理pH下的等摩尔混合物，除了在pH 9下获得的对应于PC：PA混合物的值。与其他半醌相比，这些系数遵循PC：PA<PC·-的顺序。无论是带电荷的还是中性的脂质膜，都没有检测到1，4-苯醌（BQ·-）进入脂质相的分配。脂膜的存在降低了BQ·-平衡浓度，在这里考虑的所有类型的膜的存在下。
Semiquinone membrane/buffer partition coefficients have been determined for 1,2-naphthosemiquinone (ONQ·-), 1,4-naphthosemiquinone (NQ·-) and two of its hydroxylated derivatives, 5,8-dihydroxy-1,4-naphthosemiquinone (NZQ·-) and 5-hydroxy-1,4-naphthosemiquinone (JQ·-) as a function of membrane charge in multilamellar vesicles of phosphatidylcholine (PC) and equimolar mixtures of this lipid and phosphatidic acid (PC:PA) and cetyltrimethylammonium bromide (PC:CTAB) at physiological pH with the exception of values corresponding to PC:PA mixtures which were obtained at pH 9. These coefficients follow the order PC:PA<PC·- as compared to the other semiquinones. No partition of 1,4-benzose-miquinone (BQ·-) into the lipid phase was detected for either charged or neutral lipid membranes. The presence of lipid membranes decreases the BQ·-equilibrium concentration in the presence of all the types of membranes considered here.