Relative significance of endothelium and internal elastic lamina in regulating the entry of macromolecules into arteries in vivo.
Relative significance of endothelium and internal elastic lamina in regulating the entry of macromolecules into arteries in vivo.
复制标题
内皮和内弹力层在调节体内大分子进入动脉中的相对意义。
DOI：
10.1161/01.res.74.1.74
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发表时间：
1994
影响因子：
20.1
通讯作者：
Chisolm,GM
中科院分区：
文献类型：
--
作者：
Penn,MS;Saidel,GM;Chisolm,GM
内弹性膜（IEL）的作用，它分隔动脉壁的内膜和中膜，作为一个限制性的屏障，大分子运动已被认为是动脉粥样硬化病变的发展或血管成形术期间再狭窄。然而，IEL的渗透系数从未在体内未受干扰的血管中被量化。使用一种新开发的技术，我们测量的浓度分布在内膜和媒体的阳离子（pI约8.5）和阴离子（pI约6.3）同工酶的44 kD大分子辣根过氧化物酶（HRP）。需要两种不同内膜分辨率的动脉壁转运数学模型来模拟浓度分布数据并估计感兴趣的参数。最佳估计的内皮细胞（PE）和IEL（PIEL）的HRP的渗透系数确定的最佳最小二乘拟合的两个模型的实验数据。这些估计（阴离子：PE = 0.050 +/- 0.021微米/分钟，PIEL = 0.146 +/- 0.082微米/分钟，n = 8;阳离子：PE = 0.034 +/- 0.018微米/分钟，PIEL = 0.110 +/- 0.047微米/分钟，n = 8）表明IEL占约25%（阴离子，26 +/- 9%;阳离子，25 +/-13%）对HRP从血液转运到动脉介质的阻力。尽管阳离子制剂的两个参数均较小，但差异不显著，且两种分子的IEL的相对作用相似。（250字处删节）
A role for the internal elastic lamina (IEL), which separates the intima and media of an artery wall, as a restrictive barrier to macromolecular movement has been suggested in atherosclerotic lesion development or restenosis during angioplasty. The permeability coefficient of the IEL, however, has never been quantified in unperturbed vessels in vivo. Using a newly developed technique, we measured the concentration distributions in both intima and media of cationic (pI approximately 8.5) and anionic (pI approximately 6.3) isozymes of the 44-kD macromolecule horseradish peroxidase (HRP). Two mathematical models of arterial wall transport differing in their resolution of the intima were required to simulate the concentration distribution data and to estimate the parameters of interest. Optimal estimates of the permeability coefficients of the endothelium (PE) and IEL (PIEL) to HRP were determined by the best least-squares fit of the two models to experimental data. These estimates (anionic: PE = 0.050 +/- 0.021 microns/min, PIEL = 0.146 +/- 0.082 microns/min, n = 8; cationic: PE = 0.034 +/- 0.018 microns/min, PIEL = 0.110 +/- 0.047 microns/min, n = 8) indicate that the IEL is responsible for approximately 25% (anionic, 26 +/- 9%; cationic, 25 +/- 13%) of the resistance to HRP transport from the blood into the arterial media. Although both parameters were less for the cationic preparation, the differences were not significant, and the relative role of the IEL was similar for both molecules.(ABSTRACT TRUNCATED AT 250 WORDS)