Intercalibration of the Acridine Orange Direct Count Method of Aquatic Bacteria
Intercalibration of the Acridine Orange Direct Count Method of Aquatic Bacteria
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吖啶橙直接计数法水生细菌的相互校准
DOI：
10.1264/microbes1986.4.89
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Kenji Kato
中科院分区：
文献类型：
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作者：
T. Nagata;T. Someya;T. Konda;Makoto Yamamoto*;K. Morikawa;M. Fukui;Nobuo Kuroda;Kazushi Takahashi;Su;Masayuki Mori;Shigehiro Araki;Kenji Kato
对日本7个实验室的10名研究人员进行了吖啶橙直接计数法(AODC)浮游细菌计数的相互校准。第一次校准是对发送给五个实验室的七名调查人员的一份淡水和两份海水样本进行的。每个研究人员根据每个实验室的常规程序，用AODC方法估计了这些样本中的细菌丰度。淡水样本的估算值相差2.3倍[变异系数(CV)，3b%]，而海水样本的估计值相差4.0和11倍(变异系数分别为46和94%)。在第二次校准中，9名研究人员在相同的光学条件下对相同AODC载玻片上的细菌进行了计数。9名调查人员对三个淡水样本的估计相差1.8-2.5倍(变异系数，24-29%)，而对于海水样本，他们的估计相差17倍(变异系数，89%)。与细菌在过滤器上的传染性分布有关的采样误差，以及区分细菌和非细菌颗粒的标准不同，被认为是细菌计数的可能差异来源。
Intercalibration for counting planktonic bacteria by the acridine orange direct count (AODC) method was made among ten investigators of seven laboratories in Japan. The first calibration was made for one freshwater and two seawater samples sent to seven investigators belonging to five laboratories. Each investigator estimated bacterial abundance in these samples by the AODC method according to routine procedures of each laboratory. For the freshwater sample the estimates differed by a factor of 2.3 [coefficient of variation (CV), 3b%], while for the seawater samples they varied greatly by factors of 4.0 and 11 (CV's, 46 and 94%). In the second calibration nine investigators counted bacteria on the same AODC slides under the same optical condition. The estimates of nine investigators for three freshwater samples differed by factors of 1.8-2.5 (CV's, 24-29%), while for a seawater sample they differed by a factor of 17 (CV, 89%). Sampling error associated with contagious distribution of bacteria on a filter, and difference in the criterion for discriminating bacteria from nonbacterial particles were considered to be possible sources of variation in counting bacteria.