Energy balance following diets of varying fat content: metabolic dysregulation in a rodent model of spinal cord contusion
Energy balance following diets of varying fat content: metabolic dysregulation in a rodent model of spinal cord contusion
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DOI：
10.14814/phy2.14207
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发表时间：
2019-08-01
影响因子：
2.5
通讯作者：
Grayson, Bernadette E.
中科院分区：
文献类型：
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作者：
Harris, Kwamie K.;Himel, Alexandra R.;Grayson, Bernadette E.
在脊髓损伤(SCI)人群中，代谢功能障碍可能会加剧。脊髓损伤模型结合代谢应激源对于了解该人群的疾病进展具有翻译相关性。在本研究中，我们使用了T10水平的大鼠胸段脊髓损伤(TSCI)模型，并给予含有9%或40%乳脂的饮食来创建一个独特的模型系统，以了解脊髓损伤后体重调节的生理学。进食28天后恢复的脊髓损伤大鼠的体质量、瘦质量和脂肪质量降低，但瘦或脂肪质量组成的百分比没有差异。在低脂饮食(LFD)或高脂饮食(HFD)12周后，服用LFD的脊髓损伤大鼠的体重增加速度与服用HFD的SCI动物的体重增加速度不同。LFD-SCI组饲料转化效率低于Sham-LFD组，而TSCI-HFD组与Sham-HFD组相当。尽管SCI大鼠仍然保持较低的瘦体重，但在研究结束时，喂高脂饮食的大鼠的体脂百分比高于喂LFD的大鼠。常量营养素选择测试表明，SCI大鼠对蛋白质的偏好明显高于Sham大鼠。代谢笼活动分析显示，TSCI大鼠的能量消耗增加，尽管运动活动减少。只有LFD-TSCI大鼠的肌肉甘油三酯和胆固醇降低。这些数据表明，在脊髓疾病进展的背景下，TSCI大鼠摄入HFD改变了代谢障碍的轨迹。
Within the spinal cord injured (SCI) population, metabolic dysfunction may be exacerbated. Models of cord injury coupled with metabolic stressors have translational relevance to understand disease progression in this population. In the present study, we used a rat model of thoracic SCI at level T10 (tSCI) and administered diets comprised of either 9% or 40% butterfat to create a unique model system to understand the physiology of weight regulation following cord injury. SCI rats that recovered on chow for 28 days had reduced body mass, lean mass, and reduced fat mass but no differences in percentage of lean or fat mass composition. Following 12 weeks on either low-fat diet (LFD) or high-fat diet (HFD), SCI rats maintained on LFD did not gain weight at the same rate as SCI animals maintained on HFD. LFD-SCI had reduced feed conversion efficiency in comparison to Sham-LFD whereas tSCI-HFD were equivalent to Sham-HFD rats. Although SCI rats still maintained lower lean body mass, by the end of the study HFD-fed rats had higher body fat percentage than LFD-fed rats. Macronutrient selection testing demonstrated SCI rats had a significant preference for protein over Sham rats. Analysis of metabolic cage activity showed tSCI rats had elevated energy expenditure, despite reduced locomotor activity. Muscle triglycerides and cholesterol were reduced only in LFD-tSCI rats. These data suggest that consumption of HFD by tSCI rats alters the trajectory of metabolic dysfunction in the context of spinal cord disease progression.