A model of the hydrodynamic behavior of irradiated DNA: dependence on molecular conformation.
A model of the hydrodynamic behavior of irradiated DNA: dependence on molecular conformation.
复制标题
受辐射 DNA 的流体动力学行为模型：对分子构象的依赖性。
DOI：
10.1002/bip.360260109
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Lange,CS
中科院分区：
文献类型：
--
作者：
Ostashevsky,JY;Lange,CS
本文提出的模型描述了DNA粘度和粘弹性对电离辐射诱导的分子击穿的响应。考虑了四种DNA构象：(1)线形链，(2)松弛环，(3)超螺旋环，(4)超螺旋线形。该模型包括用于计算DNA分子作为剂量函数的尺寸分布的微分方程组。这些分布被用于通常的聚合物分子非均相密度的流体动力学公式中。粘弹法测定D37的敏感性好于粘度法。该模型预测了线形和圆形DNA的辐射响应有相当大的差异。T4DNA的实验数据证实了这一预测[CS Lange等人(1984)Rad]。Res.100，1-15]和E.ColiDNA[SE Bresler et al.(1984)BiPhys.J.45,749-754]。因此，电离辐射可以用来确定DNA分子的原始构象。
The model presented here describes the DNA viscosity and viscoelastometric responses to ionizing radiation-induced molecular breakdown. Four DNA conformations are considered:(1) linear chain,(2) relaxed circle,(3) supercoiled circle, and (4) supercoiled linear. The model includes the systems of differential equations for calculating the size distributions of DNA molecules as functions of dose. Thedistributions are used in the usual hydrodynamic formulae for heterogeneouspopulations of polymer molecules. The sensitivity of viscoelastometry for the estimation of D 37 is better than that of viscometry. The model predicts a considerable difference between the radiation responsesof linear and circular DNAs. The prediction is confirmed by experimental data for T4 DNA [CS Lange et al.(1984) Rad. Res. 100, 1–15] and E. coli DNA [SE Bresler et al.(1984) Biophys. J. 45, 749–754]. Thus, ionizing radiation can be used to determine the original conformation of DNA molecules.