SENSITIVE FLUORESCENCE ASSAY FOR SIMULTANEOUS AND SEPARATE DETERMINATION OF ARYLSULPHATASE-A AND ARYLSULPHATASE-B
SENSITIVE FLUORESCENCE ASSAY FOR SIMULTANEOUS AND SEPARATE DETERMINATION OF ARYLSULPHATASE-A AND ARYLSULPHATASE-B
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DOI：
10.1016/0009-8981(77)90172-3
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发表时间：
1977-01-01
影响因子：
5
通讯作者：
SANDHOFF, K
中科院分区：
文献类型：
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作者：
CHRISTOMANOU, H;SANDHOFF, K
基于Ag+对两种酶的不同影响，利用4-甲基伞形叶硫酸酯建立了一种灵敏的荧光测定法，用于同时测定白细胞[人]中的芳基磺化酶A和B。该程序允许区分正常病例和异色性脑白质营养不良患者。
A sensitive fluorometric assay utilizing 4-methylumbelliferyl sulfate was developed for the simultaneous determination of arylsulfatases A and B from leukocytes [human], based on the differential effect of Ag+ on the 2 enzymes. The procedure allows discrimination between normal cases and those with metachromatic leukodystrophy.