SENSITIVE ENZYME-AMPLIFIED ELECTRICAL IMMUNOASSAY FOR PROTEIN A-BEARING STAPHYLOCOCCUS-AUREUS IN FOODS
SENSITIVE ENZYME-AMPLIFIED ELECTRICAL IMMUNOASSAY FOR PROTEIN A-BEARING STAPHYLOCOCCUS-AUREUS IN FOODS
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DOI：
10.1128/aem.56.11.3278-3284.1990
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发表时间：
1990-11-01
影响因子：
4.4
通讯作者：
KROLL, RG
中科院分区：
文献类型：
--
作者：
BROOKS, JL;MIRHABIBOLLAHI, B;KROLL, RG
建立了检测金黄色葡萄球菌蛋白A的电化学安培免疫分析法。该方法基于夹心免疫吸附测定，并结合了酶扩增步骤，使用NAD特异性氧化还原循环产生NADH（C。H. Stanley，A. Johannsson和C. H. Self，J. Immunol. Methods 83：89-95，1985）。还原的介质，铁氰化物，是依赖于抗原的初始浓度。通过使用相对于Ag/AgCl参比电极以+0.8V极化的Pt盘电极测量最终电势。该测定是快速的（4小时），产生的蛋白A-和细胞（S。金黄色葡萄球菌）依赖的信号。该系统灵敏度高，可检测10 pg蛋白A ml-1，
An amperometric electrochemical immunoassay specific for protein A-bearing Staphylococcus aureus was developed. The method was based on a sandwich immunosorbent assay and incorporated an enzyme amplification step, using a NAD-specific redox cycle generating NADH (C. H. Stanley, A. Johannsson, and C. H. Self, J. Immunol. Methods 83:89-95, 1985). Reduction of the mediator, ferricyanide, was dependent on the initial concentration of antigen. The final potential was measured by using a Pt disk electrode polarized at +0.8 V to the Ag/AgCl reference electrode. The assay was rapid (4 h) an generated protein A- and cell (S. aureus)-dependent signals. The system was highly sensitive and could detect 10 pg of protein A ml-1 and