GLUTATHIONE AND ELICITATION OF THE PHYTOALEXIN RESPONSE IN LEGUME CELL-CULTURES
GLUTATHIONE AND ELICITATION OF THE PHYTOALEXIN RESPONSE IN LEGUME CELL-CULTURES
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DOI：
10.1007/bf00195343
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发表时间：
1991-01-01
期刊：
影响因子：
4.3
通讯作者：
DIXON, RA
中科院分区：
文献类型：
--
作者：
EDWARDS, R;BLOUNT, JW;DIXON, RA
还原型谷胱甘肽（GSH）和氧化型谷胱甘肽引起的植物抗毒素反应的细胞悬浮培养的豆类（菜豆L。）而对紫花苜蓿（Medicago sativa L.）在菜豆细胞中，高谷胱甘肽（HGSH）是主要的可溶性巯基，用真菌诱导子处理细胞导致HGSH的积累，但不导致GSH的积累。与此相反，GSH是更丰富的比HGSH在未引发的苜蓿细胞，和细胞内水平的硫醇增加响应真菌诱导剂。用GSH生物合成的人工前体L-oxothiazolidine-4-carboxylate处理菜豆或苜蓿细胞，以与用真菌诱导剂处理后观察到的类似方式增加细胞内硫醇，但没有导致培养物的诱导。外源性[S-35]GSH在菜豆和苜蓿中的初始代谢命运存在差异，但我们的数据尚未提供解释GSH如何作为激发子的基础。我们的结论是，观察到的细胞内硫醇暴露于真菌激发子后的变化是一个结果，而不是一个原因的初始激发信号（S）。
Both reduced glutathione (GSH) and oxidized glutathione elicited the phytoalexin response in cell-suspension cultures of bean (Phaseolus vulgaris L.) but had no effect in those of alfalfa (Medicago sativa L.). In bean cells, homoglutathione (HGSH) was the predominant soluble thiol and treatment of cells with fungal elicitor resulted in the accumulation of HGSH but not GSH. In contrast, GSH was more abundant than HGSH in unelicited alfalfa cells, and the intracellular levels of both thiols increased in response to fungal elicitor. Treatment of bean or alfalfa cells with L-oxothiazolidine-4-carboxylate, an artificial precursor for GSH biosynthesis, increased intracellular thiols in an analogous manner to that observed following treatment with fungal elicitor, but did not result in elicitation of the cultures. Differences were observed in the initial metabolic fates of exogenously supplied [S-35]GSH in bean and alfalfa, but our data do not yet provide a basis for explaining how GSH acts as an elicitor. We conclude that the changes observed in intracellular thiols following exposure of cells to fungal elicitor are a consequence rather than a cause of the initial elicitation signal(s).