Electrophysiology of cultured parathyroid cells from the goat.
Electrophysiology of cultured parathyroid cells from the goat.
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山羊培养的甲状旁腺细胞的电生理学。
DOI：
10.1111/j.1748-1716.1981.tb06859.x
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
K. Hove
中科院分区：
文献类型：
--
作者：
O. Sand;S. Ozawa;K. Hove
培养的山羊甲状旁腺（PT）细胞的膜电位和电阻为44.7 +/- 6.8 mV和503 +/- 218 M Ω（平均值+/- S.D.，n = 24）。0.8 mM Ca 2+溶液中的相应值为29.2 +/- 12.2 mV和290 +/- 198 M Ω（n = 22）。电流/电压关系表明，一个明显的外向整流膜电位更积极的-40 mV。电流注入在一半的穿透细胞中产生分级的再生反应。再生反应也得到了在Na+-无溶液中，这些反应可逆地抑制钙离子阻滞剂D-600。这些结果支持PT细胞具有电压敏感性K+和Ca ~（2+）通道的观点，并且储存的PT激素的胞吐依赖于细胞外Ca ~（2+）的流入，就像在其他分泌细胞中一样。
The membrane potential and resistance of goat parathyroid (PT) cells in culture were 44.7 +/- 6.8 mV and 503 +/- 218 M omega (mean +/- S.D., n = 24) in 2.4 mM Ca2+ solution. The corresponding values in 0.8 mM Ca2+ solution were 29.2 +/- 12.2 mV and 290 +/- 198 M omega (n = 22). The current/voltage relation showed a pronounced outward rectification for membrane potentials more positive than --40 mV. Current injection produced graded, regenerative responses in half of the penetrated cells. Regenerative responses were also obtained in Na+-free solution, and these responses were reversibly inhibited by the Ca2+ blocker D-600. The results support the notion that PT cells possess voltage sensitive K+ and Ca2+ channels, and that exocytosis of stored PT hormone depends on the influx of extracellular Ca2+ as in other secretory cells.