In vivo FM:: using conventional fluorescence microscopy to monitor retinal neuronal death in vivo
In vivo FM:: using conventional fluorescence microscopy to monitor retinal neuronal death in vivo
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DOI：
10.1016/s0166-2236(02)02246-4
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发表时间：
2002-09-01
影响因子：
15.9
通讯作者：
Naskar, R
中科院分区：
文献类型：
--
作者：
Thanos, S;Indorf, L;Naskar, R
成熟视网膜神经元的创伤后死亡发生在青光眼和视神经损伤后。死亡是一个动态过程，可以通过监测随时间变化的方法进行全面分析。我们将逆行可运输荧光染料的开发与传统落射荧光显微镜的改进相结合，以在体内操纵和可视化大鼠视网膜神经元。该方法是一个相对较新的概念，有潜力监测视网膜状况，例如青光眼或视神经横断，以及在不久的将来评估神经保护策略。
Post-traumatic death of mature retinal neurons occurs in glaucoma and after optic nerve injury. The death is a dynamic process that can be fully analyzed with methods that monitor changes over time. We have coupled the development of retrogradely transportable fluorescent dyes with modification of conventional epifluorescence microscopy to manipulate and visualize rat retinal neurons in vivo. The method is a relatively new concept and has potential for the monitoring of retinal conditions, such as glaucoma or optic nerve transection, and for evaluation of neuroprotective strategies in the near future.