Expression and localization of K channels KCNQ2 and KCNQ3 in the mammalian cochlea.
Expression and localization of K channels KCNQ2 and KCNQ3 in the mammalian cochlea.
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DOI：
10.1159/000158538
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发表时间：
2009
影响因子：
1.6
通讯作者：
Jarlebark L
中科院分区：
文献类型：
--
作者：
Jin Z;Liang GH;Cooper EC;Jarlebark L
KCNQ1 和 KCNQ4 电压门控钾通道亚基在听力中发挥关键作用。 KCNQ 系列的其他成员也编码慢速、低电压激活的 K+ M 电流。我们之前报道过感觉毛细胞中存在 M 样 K+ 电流，以及耳蜗中 Kcnq 家族基因的表达。在这里，我们描述了耳蜗中 M 通道亚基 KCNQ2 和 3 的 Kcnq2/3 基因表达和分布。通过RT-PCR，我们发现Kcnq2在耳蜗和柯蒂氏器中表达，而Kcnq3在耳蜗侧壁中也有表达。在大鼠耳蜗中发现了 Kcnq2 基因的五种选择性剪接变体，其中一种以前未曾报道过。在螺旋神经节听觉神经元中观察到 KCNQ2 和 KCNQ3 免疫反应性。此外，支配毛细胞的神经纤维的无髓鞘部分和毛细胞下的突触区域显示出 KCNQ2 免疫反应性。 KCNQ3 免疫反应性在螺旋神经节卫星细胞中也很突出。这些发现表明，耳蜗 M 通道在调节细胞兴奋性和维持听觉系统中耳蜗 K+ 稳态方面发挥着重要作用。
KCNQ1 and KCNQ4 voltage-gated potassium channel subunits play key roles in hearing. Other members of the KCNQ family also encode slow, low voltage-activated K+ M currents. We have previously reported the presence of M-like K+ currents in sensory hair cells, and expression of Kcnq family genes in the cochlea. Here, we describe Kcnq2/3 gene expression and distribution of M channel subunits KCNQ2 and 3 in the cochlea. By using RT-PCR, we found expression of Kcnq2 in modiolus and organ of Corti, while Kcnq3 expression was also detected in the cochlear lateral wall. Five alternative splice variants of the Kcnq2 gene, one of which has not been reported previously, were identified in the rat cochlea. KCNQ2 and KCNQ3 immunoreactivities were observed in spiral ganglion auditory neurons. In addition, the unmyelinated parts of the nerve fibers innervating hair cells and synaptic regions under hair cells showed KCNQ2 immunoreactivity. KCNQ3 immunoreactivity was also prominent in spiral ganglion satellite cells. These findings suggest that cochlear M channels play important roles in regulation of cellular excitability and maintenance of cochlear K+ homeostasis in the auditory system.
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影响因子：
1.4
作者：
Lee, WL;Biervert, C;Steinlein, OK
通讯作者：
Steinlein, OK
影响因子：
30.8
作者：
Charlier, C;Singh, NA;Leppert, M
通讯作者：
Leppert, M
影响因子：
5.3
作者：
Beisel, KW;Rocha-Sanchez, SM;Fritzsch, B
通讯作者：
Fritzsch, B
影响因子：
11.4
作者：
Kharkovets, T;Dedek, K;Jentsch, TJ
通讯作者：
Jentsch, TJ
DOI：
10.1159/000085439
发表时间：
2005-01-01
期刊：
ORL-JOURNAL FOR OTO-RHINO-LARYNGOLOGY AND ITS RELATED SPECIALTIES
影响因子：
--
作者：
Liang, GH;Moore, EJ;Järlebark, L
通讯作者：
Järlebark, L