Control of coleopteran insect pests through RNA interference
Control of coleopteran insect pests through RNA interference
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DOI：
10.1038/nbt1359
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发表时间：
2007-11-01
影响因子：
46.9
通讯作者：
Roberts, James
中科院分区：
文献类型：
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作者：
Baum, James A.;Bogaert, Thierry;Roberts, James
用于控制作物上的鳞翅目和鞘翅目害虫的商业生物技术解决方案依赖于苏云金芽孢杆菌杀虫蛋白的表达(1,2)，其中大部分可透化易感昆虫的肠道上皮细胞膜(3)。然而，涉及不同作用模式的昆虫控制策略对于管理昆虫抗性的出现将是有价值的。为此，我们证明，摄入人工饮食中提供的双链 (ds)RNA 会引发几种鞘翅目物种的 RNA 干扰，尤其是西部玉米根虫 (WCR) Diabrotica virgifera virgifera LeConte。这可能导致幼虫发育迟缓和死亡。经工程改造表达 WCR dsRNA 的转基因玉米植物在生长室测定中显示 WCR 摄食损伤显着减少，表明可以利用 RNAi 途径通过植物内表达 dsRNA 来控制害虫。
Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins(1,2), most of which permeabilize the membranes of gut epithelial cells of susceptible insects(3). However, insect control strategies involving a different mode of action would be valuable for managing the emergence of insect resistance. Toward this end, we demonstrate that ingestion of doublestranded (ds)RNAs supplied in an artificial diet triggers RNA interference in several coleopteran species, most notably the western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte. This may result in larval stunting and mortality. Transgenic corn plants engineered to express WCR dsRNAs show a significant reduction in WCR feeding damage in a growth chamber assay, suggesting that the RNAi pathway can be exploited to control insect pests via in planta expression of a dsRNA.