Testosterone pretreatment enhances cadmium-induced increases in c-myc and c-jun transcript levels in 16 myoblasts
Testosterone pretreatment enhances cadmium-induced increases in c-myc and c-jun transcript levels in 16 myoblasts
复制标题
睾酮预处理可增强镉诱导的 16 种成肌细胞中 c-myc 和 c-jun 转录水平的增加
DOI：
10.1080/107691899229098
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
J. Hochadel
中科院分区：
文献类型：
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作者：
H. Shimada;W. Achanzar;M. Waalkes;J. Hochadel
镉是一种已知的致癌物，经常以男性生殖组织为目标。在许多细胞中，镉诱导原癌基因c-myc和c-jun的表达。几种类固醇激素可以改变镉的影响，包括睾酮。因此，我们研究了睾酮对镉毒性或L6细胞c-myc和c-jun表达的影响。无毒性剂量（0-10 muM）的睾酮不会改变镉诱导的细胞毒性或金属硫蛋白的表达，金属硫蛋白是一种参与获得性镉耐受的蛋白质。镉（5mum）使c-myc和c-jun -m RNA水平分别提高了对照的3.1倍和2.8倍。单独睾酮（10 muM）降低c-myc转录本的基础水平，但不影响c-jun。尽管睾酮预处理本身降低了转录物水平，但它使镉诱导的c-myc转录物积累量比对照增加了5倍。睾酮预处理也增强了镉诱导的c-jun mRNA积累。因此，睾酮似乎增加了镉……
Cadmium is a known carcinogen that often targets male reproductive tissues. In many cells cadmium induces expression of the proto-oncogenes c-myc and c-jun. Several steroid hormones can modify the effects of cadmium, including testosterone. Thus, we studied the effects of testosterone on cadmium toxicity or c-myc and c-jun expression in L6 cells. Testosterone at non-toxic doses (0-10 muM) did not modify either cadmium-induced cytotoxicity or expression of metallothionein, a protein involved in acquired cadmium tolerance. Cadmium (5 muM) increased c-myc and c-jun m RNA levels by 3.1-fold and 2.8-fold of control, respectively. Testosterone alone (10 muM) decreased basal levels of c-myc transcript but did not affect c-jun. Despite reducing transcript levels by itself, testosterone pretreatment increased cadmium-induced c-myc transcript accumulation to 5-fold over control. Testosterone pretreatment also enhanced cadmium induction of c-jun mRNA accumulation. Thus, it appears that testosterone increases cadmium...