Tenascin differentiates dermatofibroma from dermatofibrosarcoma protuberans: Comparison with CD34 and factor XIIIa
Tenascin differentiates dermatofibroma from dermatofibrosarcoma protuberans: Comparison with CD34 and factor XIIIa
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DOI：
10.1053/hupa.2001.21137
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
From, L
中科院分区：
文献类型：
--
作者：
Kahn, HJ;Fekete, E;From, L
皮肤纤维瘤与隆突性皮肤纤维肉瘤的鉴别很困难。CD34和因子XIIIa已被用于区分DF和DFSP。然而，它们的表达存在重叠和缺乏特异性。腱生蛋白是一种细胞外基质糖蛋白，参与胚胎发生、癌发生和伤口愈合。该研究的目的是评估腱生蛋白在DF和DFSP中的作用，并将结果与用CD 34和因子XIIIa获得的结果进行比较。免疫组织化学染色进行了20例DFSP和DF，使用抗体tenascin，CD34和因子XIIIa，和链霉亲和素生物素技术。在肿瘤内评估所有3种抗体的阳性。还评估了真皮-表皮交界处的腱生蛋白表达。在20例DF中，20例（100%）在真皮-表皮交界处观察到强阳性表达，而在20例DFSP中，20例（100%）在病变上方为阴性。80%的DF和DFSP（16/20例）的病灶内可见Tenascin。CD34在20例DFSP中的16例（80%）和20例DF中的5例（25%）中强表达，而因子XIIIa在20例DF中的19例（95%）和15例DFSP中的3例（15%）中强表达。尽管CD34在80%的DFSP中表达，而因子XIIIa在95%的DF中表达，但它们在两种类型的肿瘤中的表达存在重叠。在DF而非DFSP的真皮-表皮上，腱生蛋白表达增加，可区分这两种肿瘤。相反，腱生蛋白在病变内的表达不能区分DF和DFSP。Copyright（C）2001 by W.B.桑德斯公司
Differentiation of dermatofibroma (DF) from dermatofibrosarcoma protuberans (DFSP) carl be difficult. CD34 and Factor XIIIa have been used to differentiate DF from DFSP. However, there is overlap and lack of specificity of their expression. Tenascin is an extracellular matrix glycoprotein that is involved in embryogenesis, carcinogenesis, and wound healing. The aim of the study was to assess the role of tenascin in DF and DFSP and compare the results with those obtained with CD34 and Factor XIIIa. Immunohistochemical staining was performed on 20 cases each of DFSP and DF, using antibodies to tenascin, CD34 and Factor XIIIa, and the streptavidin biotin technique. Positivity for all 3 antibodies was assessed within the tumors. Tenascin expression was also assessed at the dermal-epidermal junction. Strong tenascin positivity was noted at the dermal-epidermal junction overlying the lesion in 20 of 20 cases of DF (100%) and was negative over the lesion in 20 of 20 cases DFSP (100%). Tenascin was noted within the lesion of 80% of both DF and DFSP (16/20 cases). CD34 was strongly expressed in 16 of 20 (80%) DFSP and 5 of 20 (25%;,) DF, whereas Factor XIIIa was strongly expressed in 19 of 20 (95%) DF and 3 of 15 (15%) DFSP. Although CD34 was expressed in 80% DFSP and Factor XIIIa in 95% of DF, there was overlap in their expression in the 2 types of tumors. The increased expression of tenascin at the dermal-epidermal overlying the lesion in DF but not in DFSP, differentiated these 2 tumors. In contrast, tenascin expression within the lesion did not differentiate DF from DFSP. Copyright (C) 2001 by W.B. Saunders Company.