Mutations induced by saturated aqueous nitric oxide in the pSP189 supF gene in human Ad293 and E. coli MBM7070 cells.
Mutations induced by saturated aqueous nitric oxide in the pSP189 supF gene in human Ad293 and E. coli MBM7070 cells.
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在人 Ad293 和大肠杆菌 MBM7070 细胞中，饱和一氧化氮水溶液诱导 pSP189supF 基因突变。
DOI：
10.1093/carcin/14.7.1251
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发表时间：
1993
期刊：
影响因子：
4.7
通讯作者：
Dipple,A
中科院分区：
文献类型：
--
作者：
Routledge,MN;Wink,DA;Keefer,LK;Dipple,A
一氧化氮是一种重要的生物调节剂，也可能是一种内源性和外源性的人类诱变剂。为了研究穿梭载体携带的靶基因暴露于一氧化氮后产生的突变，通过在需氧缓冲溶液中用一氧化氮处理，然后在人Ad 293或大肠杆菌MBM 7070细胞中复制质粒，在pSP 189穿梭载体的supF基因中诱导突变。诱导的突变频率随一氧化氮剂量增加而增加，当氧化吸收的一氧化氮总量为100 mmol/l含有质粒的pH 7.4缓冲液时，诱导的突变频率比人细胞中的自发背景高44倍，比细菌细胞中的背景高>15倍。分析的大多数点突变（人和E.大肠杆菌细胞）是AT→GC转换，GC→AT转换（29%和23%）是第二最普遍的。在两种细胞类型中观察到的supF基因的各种点突变的总体频率相似，尽管热点的分布显示出差异。结果与DNA碱基脱氨基引发的突变机制一致。
Nitric oxide is an important bioregulatory agent that may also be an endogenous and exogenous human mutagen. In order to study mutations generated following exposure of a shuttle vector-borne target gene to nitric oxide, mutations were induced in thesupFgene of the pSP189 shuttle vector by treatment with nitric oxide in aerobic buffered solution followed by replication of the plasmid in either human Ad293 orEscherichia coliMBM7070 cells. The induced mutation frequency, which increased with nitric oxide dose, was 44-fold greater than the spontaneous background in human cells and >15-fold greater than background in the bacterial cells when a total of 100 mmol of nitric oxide was oxidatively absorbed/1 of pH 7.4 buffer containing the plasmid. The majority of point mutations analysed (61 and 75% for human andE. colicells respectively) were AT→GC transitions with GC→AT transitions (29 and 23%) being the next most prevalent. The overall frequencies of the various point mutations seen in thesupFgene were similar in the two cell types, although the distribution of hotspots showed differences. The results are consistent with a mutational mechanism initiated by deamination of DNA bases.