INACTIVATION OF PROTEASE INHIBITOR PHENYLMETHYLSULFONYL FLUORIDE IN BUFFERS
INACTIVATION OF PROTEASE INHIBITOR PHENYLMETHYLSULFONYL FLUORIDE IN BUFFERS
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DOI：
10.1016/0003-2697(78)90784-4
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发表时间：
1978-01-01
影响因子：
2.9
通讯作者：
JAMES, GT
中科院分区：
文献类型：
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作者：
JAMES, GT
苯甲基磺酰氟 (PMSF) 的水性制剂对蛋白酶失去活性，除非立即与蛋白酶接触。 PMSF 的失活随着 pH 值和温度的升高而增加。抑制剂的半衰期为 25°C。在 pH 7.0、7.5 和 8.0 时，C 分别约为 110、55 和 35 分钟。在 pH 8 时，100 μM PMSF 在 25°C 下 1 小时内几乎完全失活。 C 或 4°C 下 22 小时内。 C. PMSF 的 100% 异丙醇储备溶液在 25°C 下稳定。 C 几个月甚至更长时间。 PMSF 抑制的胰凝乳蛋白酶在 25°C 下 1 周内未发生重新激活。 pH 7.0 时 C。
Aqueous preparations of phenylmethylsulfonyl fluoride (PMSF) become inactive toward proteases unless promptly brought into contact with protease. Inactivation of PMSF increases with increased pH and temperature. Half-lives of the inhibitor at 25.degree. C are approximately 110, 55 and 35 min at pH 7.0, 7.5 and 8.0, respectively. At pH 8, 100 .mu.M PMSF is almost completely inactivated within 1 h at 25.degree. C or within 22 h at 4.degree. C. Stock solutions of PMSF in 100% isopropanol are stable at 25.degree. C for months if not longer. Reactivation of PMSF-inhibited chymotrypsin did not occur within 1 wk at 25.degree. C at pH 7.0.