AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea
AtTIP1;3 and AtTIP5;1, the only highly expressed Arabidopsis pollen-specific aquaporins, transport water and urea
复制标题
DOI：
10.1016/j.febslet.2008.11.002
复制
发表时间：
2008-12-10
期刊：
影响因子：
3.5
通讯作者：
Muschietti, Jorge P.
中科院分区：
文献类型：
--
作者：
Soto, Gabriela;Alleva, Karina;Muschietti, Jorge P.
Polymorphism包括水和/或溶质运动必须精细调节的过程，表明水通道蛋白的参与。利用拟南芥成熟花粉不同转录谱的信息，我们发现，只有水通道蛋白，选择性和高度表达的成熟花粉是两个TIP：AtTIP 1;3和AtTIP 5;1。与其他单细胞转录谱相比，花粉中水通道蛋白表达基因的数量较少，且类型更独特。当其特征在于使用爪蟾卵母细胞肿胀试验，AtTIP 1;3和AtTIP 5;1显示中间的水渗透性。虽然它们既不显示甘油也不显示硼酸渗透性，但它们都运输尿素。综上所述，这些结果表明AtTIP 1;3和AtTIP 5;1在拟南芥花粉中作为特异性的水和尿素通道发挥作用。(C)2008年欧洲生化学会联合会。Elsevier B. V.出版，保留所有权利。
Pollination includes processes where water and/or solute movements must be finely regulated, suggesting participation of aquaporins. Using information available from different transcriptional profilings of Arabidopsis thaliana mature pollen, we showed that the only aquaporins that are selectively and highly expressed in mature pollen are two TIPs: AtTIP1;3 and AtTIP5;1. Pollen exhibited a lower number and more exclusive type of aquaporin expressed genes when compared to other single cell transcriptional profilings. When characterized using Xenopus oocyte swelling assays, AtTIP1;3 and AtTIP5;1 showed intermediate water permeabilities. Although they displayed neither glycerol nor boric acid permeability they both transported urea. In conclusion, these results suggest a function for AtTIP1;3 and AtTIP5;1 as specific water and urea channels in Arabidopsis pollen. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.