Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish.
Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish.
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DOI：
10.1002/dvdy.22567
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发表时间：
2011-03
影响因子：
2.5
通讯作者：
Link, Brian A.
中科院分区：
文献类型：
--
作者：
Collery, Ross F.;Link, Brian A.
关键词：
BMP 信号传导对于发育、损伤反应和体内平衡至关重要。我们开发了转基因斑马鱼，可以在胚胎和成体中报告 Smad 介导的 BMP 信号传导。这些细胞系在充分表征的“BMP 响应元件”(BRE) 下表达 eGFP、不稳定的 eGFP 或不稳定的 KO2。这些荧光蛋白被发现在已知 BMP 信号传导区域动态表达，包括发育中的尾芽、造血谱系、背眼、大脑结构、心脏、下巴、鳍和体节以及其他组织。通过观察 hsp70:bmp2b 转基因背景中激活后的荧光或通过 hsp70:nog3 背景中的抑制来确认对 BMP 信号传导变化的响应性。我们进一步证明了使用 BMP 受体活性抑制剂 Dorsomorphin 对 BMP 信号进行化学抑制后，BRE 转基因品系的忠实报道。总体而言，这些细胞系将作为有价值的工具来探索胚胎发生、组织维持和疾病期间 BMP 信号的机制和调节。
BMP signaling is fundamental to development, injury response, and homeostasis. We have developed transgenic zebrafish that report Smad-mediated BMP signaling in embryos and adults. These lines express either eGFP, destabilized eGFP, or destabilized KO2 under the well-characterized ‘BMP Response Element’ (BRE). These fluorescent proteins were found to be expressed dynamically in regions of known BMP signaling including the developing tailbud, hematopoietic lineage, dorsal eye, brain structures, heart, jaw, fins, and somites, as well as other tissues. Responsiveness to changes in BMP signaling was confirmed by observing fluorescence after activation in an hsp70:bmp2b transgenic background or by inhibition in an hsp70:nog3 background. We further demonstrated faithful reportage by the BRE transgenic lines following chemical repression of BMP signaling using an inhibitor of BMP receptor activity, dorsomorphin. Overall, these lines will serve as valuable tools to explore the mechanisms and regulation of BMP signal during embryogenesis, in tissue maintenance, and during disease.
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