THE CONVERSION OF HEPATITIS-B CORE ANTIGEN SYNTHESIZED IN ESCHERICHIA-COLI INTO E-ANTIGEN
THE CONVERSION OF HEPATITIS-B CORE ANTIGEN SYNTHESIZED IN ESCHERICHIA-COLI INTO E-ANTIGEN
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DOI：
10.1002/jmv.1890080404
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发表时间：
1981-01-01
影响因子：
12.7
通讯作者：
MURRAY, K
中科院分区：
文献类型：
--
作者：
MACKAY, P;LEES, J;MURRAY, K
乙型肝炎病毒e抗原(HBeAg)是一种17-20,000道尔顿的多肽，与Dane颗粒的核心抗原(HBcAg)密切相关，通过各种破坏性步骤从Dane颗粒中释放出来。HBeAg可能是HBVc核心颗粒的独特成分，也可能是HBcAg的衍生物。为了解决这一问题，用在大肠杆菌中合成的HBcAg制剂进行了免疫扩散实验，该重组质粒可表达HBcAg，但不表达其他乙肝病毒基因。在解离条件下，HBcAg通过蛋白质降解转化为HBeAg，从而在分子水平上证实了HBeAg是HBcAg的组成部分。这为检测HBeAg和抗HBeAg抗体提供了新的途径。
The e antigen (HBeAg) of hepatitis B virus (HBV) is a polypeptide of 17-20,000 daltons closely associated with the core antigen (HBcAg) of Dane particles, from which it is released by a variety of disruptive procedures. HBeAg could be a unique component of HBV core particles or a derivative of HBcAg. To resolve this question immunodiffusion experiments were carried out with preparations of HBcAg synthesized in E. coli carrying a recombinant plasmid from which the HBcAg, but no other HBV gene, was expressed. HBcAg was converted into HBeAg by proteolytic degradation under dissociating conditions, thus confirming at the molecular level that HBeAg is a component of HBcAg. This offers a new route to the detection of HBeAg and antibodies to the antigen.