Twenty-three generations of mice bidirectionally selected for open-field thigmotaxis:: Selection response and repeated exposure to the open field
Twenty-three generations of mice bidirectionally selected for open-field thigmotaxis:: Selection response and repeated exposure to the open field
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DOI：
10.1016/j.beproc.2005.11.010
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发表时间：
2006-03-01
影响因子：
1.3
通讯作者：
Ewalds-Kvist, SBM
中科院分区：
文献类型：
--
作者：
Leppänen, PK;Ravaja, N;Ewalds-Kvist, SBM
我们研究了：(a) 23代小鼠对开放场（OF）趋近性双向选择的反应，(b)反复暴露（5天）对选择性培育的高趋近性（HOFT）和低趋近性（LOFT）小鼠不同的OF行为的影响。该研究共使用了2049只小鼠。在选择实验测试之前，连续4天，每天将小鼠暴露在OF仪器中约2分钟。因此，选择是基于4个习惯期后第5天登记的分数。在几乎每一代中，hft小鼠都比LOFT小鼠更具移动性。此外，高情商小鼠比低情商小鼠更倾向于培养孩子，这可以用情绪与探索倾向之间的反比关系来解释。这些线在移动上一般没有差别。性别差异存在于移动、行走和饲养方面。在重复暴露实验中，研究了在5天的测试中9种不同of行为的发展。两条线在第1、4和5天移动、探索和隆起最多。毛发梳理和径向潜伏期在测试期间呈线性下降，心脏趋势性呈线性上升。在行走、探索、梳理和饲养方面存在行差异，而在行走和探索方面存在性别差异。株系间趋向性差异仅在第5天表现明显。时间变化部分地与一般假设不符。研究发现，趋近性是产生两种不同系小鼠的一个强有力的特征。(c) 2005 Elsevier B.V.版权所有
We examined: (a) the response to bidirectional selection for open-field (OF) thigmotaxis in mice for 23 generations and (b) the effects of repeated exposure (during 5 days) on different OF behaviors in the selectively bred high OF thigmotaxis (HOFT) and low OF thigmotaxis (LOFT) mice. A total of 2049 mice were used in the study. Prior to the testing in the selection experiment, the mice were exposed to the OF apparatus for approximately 2 min on each of 4 consecutive days. Thus, the selection was based on the scores registered on the 5th day after the four habituation periods. The HOFT mice were more thigmotactic than the LOFT mice in almost each generation. The HOFT mice also tended to rear less than the LOFT mice, which was explained by the inverse relationship between emotionality and exploratory tendencies. The lines did not generally differ in ambulation. Sex differences were found in thigmotaxis, ambulation, and rearing. In the repeated exposure experiment, the development of nine different OF behaviors across the 5 days of testing was addressed. Both lines ambulated, explored, and reared most on the 1st, 4th, and 5th days. Grooming and radial latency decreased and thigmotaxis increased linearly across the testing days. Line differences were found in ambulation, exploration, grooming, and rearing, while sex differences were manifested in ambulation and exploration. The line difference in thignnotaxis was evident only on the 5th day. Temporal changes were partially at variance with the general assumptions. OF thigmotaxis was found to be a powerful characteristic for producing two diverging lines of mice. (c) 2005 Elsevier B.V. All rights reserved.