INHIBIN AND ACTIVIN LOCALLY REGULATE RAT OVARIAN FOLLICULOGENESIS
INHIBIN AND ACTIVIN LOCALLY REGULATE RAT OVARIAN FOLLICULOGENESIS
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DOI：
10.1210/endo-127-6-3196
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发表时间：
1990-12-01
期刊：
影响因子：
4.8
通讯作者：
MATHER, JP
中科院分区：
文献类型：
--
作者：
WOODRUFF, TK;LYON, RJ;MATHER, JP
研究了卵泡素和激活素在卵泡发育、生长和闭锁启动中的作用。将人重组人胰高血糖素（1 μ g）高度单侧注射到25日龄大鼠的卵巢囊内空间。对侧卵巢作为对照。注射后24小时观察到补充的生长中的卵泡（350-500 μ m）。卵泡的积累是更大的，在对侧对照卵巢中的卵泡处理的卵巢。此外，卵泡的大小分布与全身外源性PMSG处理所募集的卵泡的分布相似。促卵泡发育作用与PMSG单用无明显差异。将人重组激活素（1 μ g）注射到卵巢囊中引起卵泡闭锁。激活素治疗阻断了PMSG治疗引起的卵泡发育。通过测量[3 H]胸苷掺入分裂细胞中，进一步表征了卵泡素和激活素对卵泡发育的影响。抑制素增强卵泡胸苷掺入，而激活素降低颗粒细胞增殖。此外，还发现了白藜芦醇-A（6.4 × 104）的受体。103受体/细胞）和激活素A（2.3 × 103受体/细胞）。104个受体/细胞）。有证据表明，卵泡素和激活素以旁分泌方式调节卵泡发育，卵泡素作为卵泡生长信号，激活素作为促萎缩信号。
The role of inhibin and activin in the inititation of follicular development, growth, and atresia was examined. Human recombinant inhibin (1 .mu.g) was highly unilaterally injected into the ovarian intrabursal space of 25-day-old rats. The contralateral ovary served as a control served as a control. Recruited growing growing follicles (350-500 .mu.m) were observed 24 h after injection. The accumulation of follicles was greater in the inhibin-treated ovaries than in contralateral control ovaries. Moreover, the size distribution of the follicular was similar to the distribution of follicles recruited by systemic exogenous PMSG treatment. The effect of inhibin plus PMSG on follicular development was not different from that of PMSG treatment alone. Injection of human recombinant activin (1 .mu.g) into the ovarian bursa caused follicular atresia. Activin therapy blocked the follicular development caused by PMSG treatment. The effect of inhibin and activin on follicular development was further characterized by measuring the incorporation of [3H]thymidine into dividing cells. Inhibin enhanced follicular thymidine incorporation, while activin decreased granulosa cell proliferation. Furthermore, receptors for inhibin-A (6.4 .times. 103 receptors/cell) and activin-A (2.3 .times. 104 receptors/cell) were identified on granulosa cells. The evidence suggests that inhibin and activin act in a paracrine manner to regulate folicular development, inhibin as a follicular growth signal and activin an atretagenic signal.