Identification of human T cell leukemia virus in a Japanese patient with adult T cell leukemia and cutaneous lymphomatous vasculitis.
Identification of human T cell leukemia virus in a Japanese patient with adult T cell leukemia and cutaneous lymphomatous vasculitis.
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一名患有成人 T 细胞白血病和皮肤淋巴瘤性血管炎的日本患者体内人类 T 细胞白血病病毒的鉴定。
DOI：
10.1073/pnas.80.7.2054
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发表时间：
1983
影响因子：
11.1
通讯作者：
Metzgar,RS
中科院分区：
文献类型：
--
作者：
Haynes,BF;Miller,SE;Palker,TJ;Moore,JO;Dunn,PH;Bolognesi,DP;Metzgar,RS
我们已经确定了一名日本成人T细胞白血病（ATL）患者，其T细胞在体外产生人类T细胞白血病病毒（HTLV）。该患者表现为淋巴瘤性关节炎和白血病，随后出现皮肤病变。恶性T细胞的皮肤浸润是血管中心性的，并产生血管壁破坏，导致坏死的皮肤肿瘤结节。在体外培养之前，外周血、皮肤和关节中的恶性T细胞不表达p19 HTLV相关抗原。然而，通过电子显微镜，在皮肤浸润性T细胞中观察到细胞内C型病毒颗粒。外周血恶性细胞培养7天后，T细胞生长因子补充培养基表达p19抗原，C型病毒颗粒被电子显微镜观察到从恶性T淋巴细胞出芽。丝裂霉素C处理的外周血T细胞诱导脐带血T细胞转化为HTLV感染的p19+ T细胞。在我们的患者的恶性T细胞中HTLV的展示证实了HTLV与日本成人T细胞白血病的关联。此外，HTLV可能与血管炎-关节炎综合征有关。
We have identified a Japanese patient with adult T-cell leukemia (ATL) whose T cells in vitro produced the human T-cell leukemia virus (HTLV). This patient presented with lymphomatous arthritis and leukemia and subsequently developed skin lesions. Skin invasion by malignant T-cells was angiocentric and produced vessel wall destruction, resulting in necrotic cutaneous tumor nodules. Malignant T cells in peripheral blood, skin, and joint prior to culture in vitro did not express p19 HTLV-associated antigen. However, by electron microscopy, intracellular type C viral particles were seen in skin-infiltrating T cells. Peripheral blood malignant cells after 7 days in culture with T-cell growth factor-supplemented media expressed p19 antigen, and type C virus particles were seen by electron microscopy to be budding from malignant T lymphocytes. Mitomycin-C-treated peripheral-blood T cells induced the transformation of cord blood T cells into HTLV-infected p19+ T cells. The demonstration of HTLV in malignant T cells from our patient confirms the association of HTLV with Japanese adult T-cell leukemia. Moreover, HTLV may be associated with a vasculitis-arthritis syndrome.