IN VITRO ACTIONS OF GROWTH HORMONE ON GLUCOSE METABOLISM IN ADIPOSE TISSUE.
IN VITRO ACTIONS OF GROWTH HORMONE ON GLUCOSE METABOLISM IN ADIPOSE TISSUE.
复制标题
生长激素对脂肪组织葡萄糖代谢的体外作用。
DOI：
10.1210/endo-76-2-216
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发表时间：
1965
期刊：
影响因子：
4.8
通讯作者：
H. Goodman
中科院分区：
文献类型：
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作者：
H. Goodman
从垂体切除大鼠获得的附睾脂肪组织对体外添加生长激素的反应是葡萄糖摄取增加、氧化为CO2和转化为脂肪酸。在这方面，发现低至0.01 μg/ml的牛生长激素有效。总共对来自5种动物的10种生长激素制剂进行了检测，发现所有制剂均具有活性。生长激素也产生这些影响时，正常大鼠脂肪组织喂养高碳水化合物，无脂肪的饮食进行了测试，但对脂肪组织喂养的标准实验室口粮的正常大鼠无效。葡萄糖利用率的增加伴随着CO2中碳1和碳6的出现成比例地增加。促肾上腺皮质激素和促甲状腺激素，而刺激葡萄糖摄取和氧化为CO2，未能增加葡萄糖碳纳入脂肪酸。其他垂体激素测试只有催产素和某些催乳素制剂引起葡萄糖代谢的变化。
Epididymal adipose tissue obtained from hypophysectomized rats responds to the addition of growth hormone in vitro with increased glucose uptake, oxidation to CO2 and conversion to fatty acids. As little as 0.01 μg/ml of bovine growth hormone was found effective in this regard. In all, 10 growth hormone preparations from 5 species of animals were tested and all were found to be active. Growth hormone also produced these effects when adipose tissues from normal rats fed a high carbohydrate, fat-free diet were tested, but was ineffective on adipose tissue from normal rats fed the standard laboratory ration. The increase in glucose utilization was accompanied by a proportionate increase in appearance of both carbon 1 and carbon 6 in CO2. Corticotropin and thyrotropin, while stimulating glucose uptake and oxidation to CO2, failed to increase the incorporation of glucose carbons into fatty acids. Of the other pituitary hormones tested only oxytocin and certain prolactin preparations caused changes in glucose m...