Activation of adenosine A2A receptors in the olfactory tubercle promotes sleep in rodents
Activation of adenosine A2A receptors in the olfactory tubercle promotes sleep in rodents
复制标题
嗅结节中腺苷 A2A 受体的激活可促进啮齿类动物的睡眠。
DOI：
10.1016/j.neuropharm.2019.107923
复制
发表时间：
2020-05-15
影响因子：
4.7
通讯作者：
Huang, Zhi-Li
中科院分区：
文献类型：
--
作者：
Li, Rui;Wang, Yi-Qun;Huang, Zhi-Li
嗅结节（olfactory tubercle，OT）是处理感觉信息的重要核团，在嗅觉刺激下可改变大脑皮层的活动。然而，关于OT在睡眠-觉醒调节中的生理作用和机制知之甚少。OT表达丰富的腺苷A（2A）受体（A（2A）Rs），其在睡眠调节中是重要的。因此，我们假设OT通过A（2A）Rs调节睡眠。本研究通过脑电图和肌电图记录与药理学和化学遗传学操作在自由移动的啮齿动物的睡眠-觉醒的配置文件。与对照组相比，通过化学遗传学激活OT A（2A）Rs和OT A（2A）R神经元分别增加非快速眼动睡眠5和3 h，而阻断A（2A）Rs减少非快速眼动睡眠。追踪和电生理研究表明，OT A（2A）R神经元投射到腹侧苍白球和外侧下丘脑，形成抑制性神经支配。总之，这些发现表明OT中的A（2A）Rs在睡眠调节中起重要作用。
The olfactory tubercle (OT), an important nucleus in processing sensory information, has been reported to change cortical activity under odor. However, little is known about the physiological role and mechanism of the OT in sleep-wake regulation. The OT expresses abundant adenosine A(2A) receptors (A(2A)Rs), which are important in sleep regulation. Therefore, we hypothesized that the OT regulates sleep via A(2A)Rs. This study examined sleep-wake profiles through electroencephalography and electromyography recordings with pharmacological and chemogenetic manipulations in freely moving rodents. Compared with their controls, activation of OT A(2A)Rs pharmacologically and OT A(2A)R neurons via chemogenetics increased non-rapid eye movement sleep for 5 and 3 h, respectively, while blockade of A(2A)Rs decreased non-rapid eye movement sleep. Tracing and electrophysiological studies showed OT A(2A)R neurons projected to the ventral pallidum and lateral hypothalamus, forming inhibitory innervations. Together, these findings indicate that A(2A)Rs in the OT play an important role in sleep regulation.