MYELIN-ASSOCIATED GLYCOPROTEIN SHARES AN ANTIGENIC DETERMINANT WITH A GLYCOPROTEIN OF HUMAN-MELANOMA CELLS
MYELIN-ASSOCIATED GLYCOPROTEIN SHARES AN ANTIGENIC DETERMINANT WITH A GLYCOPROTEIN OF HUMAN-MELANOMA CELLS
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DOI：
10.1111/j.1471-4159.1986.tb00795.x
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发表时间：
1986-11-01
影响因子：
4.7
通讯作者：
QUARLES, RH
中科院分区：
文献类型：
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作者：
NORONHA, AB;HARPER, JR;QUARLES, RH
硫酸化的100 K-道尔顿糖蛋白已被证明释放到黑色素瘤细胞的培养基中。针对人黑素瘤细胞产生的单克隆抗体10 C5和11B 5以及HNK-1与该糖蛋白结合。这里显示，针对人MAG和与神经病变相关的人IgM副蛋白的小鼠抗髓鞘相关糖蛋白（MAG）碳水化合物抗体也结合到相同的100 K分子。然而，识别肽表位的抗MAG抗体似乎不与黑素瘤细胞的这种糖蛋白反应，结果表明其与MAG的相似性仅限于共享的碳水化合物部分。抗黑色素瘤抗体（10 C5和11B 5）与HNK-1相似，与MAG和一些19- 28 K道尔顿糖蛋白以及周围神经系统（PNS）的硫酸化含葡萄糖醛酸鞘糖脂结合。此外，抗黑色素瘤抗体与神经细胞粘附分子（N-CAM）交叉反应，这一观察结果强调了MAG和其他粘附分子之间的共同抗原性。结果表明，抗黑素瘤抗体属于一类单克隆抗体（包括HNK-1、与神经病变相关的人IgM副蛋白、抗人MAG抗体和L2抗体），其特征在于对人MAG、N-CAM和PNS的几种蛋白质和脂质糖缀合物共有的相关碳水化合物决定簇的反应性。
A sulfated 100K-dalton glycoprotein has been shown to be released into the culture medium of melanoma cells. Monoclonal antibodies 10C5 and 11B5, which were raised to human melanoma cells, as well as HNK-1 bind to this glycoprotein. It is shown here that mouse anti-myelin-associated glycoprotein (MAG) carbohydrate antibodies raised to human MAG and a human IgM paraprotein associated with neuropathy also bind to the same 100K molecule. However, anti-MAG antibodies recognizing peptide epitopes do not appear to react with this glycoprotein of melanoma cells, a result suggesting that its similarity to MAG is restricted to shared carbohydrate moieties. The anti-melanoma antibodies (10C5 and 11B5) resemble HNK-1 in binding to MAG and to some 19-28K-dalton glycoproteins and sulfated, glucuronic acid-containing sphingoglycolipids of the peripheral nervous system (PNS). In addition, the anti-melanoma antibodies cross-react with neural cell adhesion molecule (N-CAM), an observation emphasizing the shared antigenicity between MAG and other adhesion molecules. The results demonstrate that the anti-melanoma antibodies fall into a class of monoclonal antibodies (including HNK-1, human IgM paraproteins associated with neuropathy, anti-human MAG antibodies, and L2 antibodies) that are characterized by reactivity against related carbohydrate determinants shared by human MAG, N-CAM, and several protein and lipid glycoconjugates of the PNS.