Macrophages in developing mammalian skeletal muscle: evidence for muscle fibre death as a normal developmental event.
Macrophages in developing mammalian skeletal muscle: evidence for muscle fibre death as a normal developmental event.
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哺乳动物骨骼肌发育中的巨噬细胞：肌纤维死亡作为正常发育事件的证据。
DOI：
10.1159/000147059
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
M. Jones
中科院分区：
文献类型：
--
作者：
E. A. Abood;M. Jones
用电镜观察了胎鼠、生长期和成年大鼠膈肌的巨噬细胞。此外，对膈肌、前腹壁肌肉和小牛肌肉的冷冻切片进行酸性磷酸酶活性染色，并在光学显微镜下进行检查。在妊娠20天和2周龄之前，经常在肌肉中观察到巨噬细胞。在4周龄大鼠中较少发现，在8周龄和成年大鼠中很少发现。它们被发现与某些电子密度很高并被认为正在退化的肌肉纤维密切接触，有时明显包围。此外，在巨噬细胞胞质内发现肌原纤维作为吞噬体。有证据支持巨噬细胞从胚胎组织中的间充质细胞发育而来的理论。在妊娠16天和18天的肌肉中发现了被认为是早期巨噬细胞并含有小溶酶体的细胞。在所有其他方面，这些细胞类似于间充质细胞。细胞死亡可能是骨骼肌发育过程中的一个正常事件，其中巨噬细胞在清除死纤维中起着重要作用。
Macrophages in the diaphragm of fetal, growing and adult rats were investigated using electron microscopy. In addition, frozen sections of the diaphragm, muscles of the anterior abdominal wall and muscles of the calf were stained for acid phosphatase activity and examined with the light microscope. Macrophages were frequently observed in the muscles at 20 days of gestation and until the animals were 2 weeks old. They were less frequently found in the 4-week-old rats and very rarely found in the 8-week-old and adult rats. They were found in intimate contact with, sometimes apparently surrounding, certain muscle fibres which were very electron dense and considered to be degenerating. In addition, myofibrils were found as phagosomes within the macrophage cytoplasm. There was evidence to support the theory that macrophages develop from mesenchymal cells in embryonic tissue. Cells considered to be early macrophages and containing small lysosomes were found in the muscles at 16 and 18 days of gestation. In all other respects these cells resembled mesenchymal cells. It is proposed that cell death may be a normal developmental event in skeletal muscle, in which macrophages play an important role in the removal of the dead fibres.