The effects of glucose and fatty acids on CXCL10 expression in skeletal muscle cells
The effects of glucose and fatty acids on CXCL10 expression in skeletal muscle cells
复制标题
DOI：
10.1080/09168451.2020.1814127
复制
发表时间：
2020-09-02
影响因子：
1.6
通讯作者：
Nedachi, Taku
中科院分区：
文献类型：
--
作者：
Ishiuchi-Sato, Yuri;Hiraiwa, Erika;Nedachi, Taku
骨骼肌产生被称为肌因子的分泌因子，其改变靶组织的生理功能。我们最近发现C-X-C趋化因子配体10 （CXCL10）是一种新的肌肉因子，在运动时下调。在本研究中，我们研究了营养变化是否会影响小鼠骨骼肌中CXCL10的表达。在饲喂正常饮食或高脂饮食10周的小鼠中评估CXCL10的表达。在喂食HFD的动物中，cxcl10的表达在快速抽搐的肌肉中被显著诱导，并伴有血糖和游离脂肪酸水平的升高。体外C2C12肌管实验表明，葡萄糖和棕榈酸水平的升高直接增强了CXCL10的表达。有趣的是，棕榈酸的作用被棕榈油酸减弱了。考虑到其强大的血管抑制活性，营养变化诱导CXCL10可能导致骨骼肌微血管网络的损伤。
Skeletal muscles produce secretory factors termed as myokines, which alter physiological functions of target tissues. We recently identified C-X-C chemokine ligand 10 (CXCL10) as a novel myokine, which is downregulated in response to exercise. In the present study, we investigated whether the nutritional changes affect CXCL10 expression in mouse skeletal muscle. Expression of CXCL10 was evaluated in mice fed a normal diet or a high fat diet for 10 weeks. In animals fed on HFD,Cxcl10expression was significantly induced in fast-twitched muscles, and was accompanied by increased blood glucose and free fatty acid levels.In vitroexperiments using C2C12 myotubes suggested that the increased levels of glucose and palmitic acids directly enhanced CXCL10 expression. Interestingly, the effect of palmitic acids was attenuated by palmitoleic acids. Considering its potent angiostatic activity, induction of CXCL10 by nutritional changes may contribute to the impairment of microvascular networks in skeletal muscles.