3D microtomographic characterization of precision extruded poly-ε-caprolactone scaffolds
3D microtomographic characterization of precision extruded poly-ε-caprolactone scaffolds
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DOI：
10.1002/jbm.b.30050
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发表时间：
2004-08-15
影响因子：
3.4
通讯作者：
Sun, W
中科院分区：
文献类型：
--
作者：
Darling, AL;Sun, W
组织工程的主要方法之一是在3D培养或植入之前用祖细胞接种可生物降解的、生物相容的聚合物支架。这些支架的微结构对细胞的附着、迁移和分化能力有直接影响。微断层扫描仪（micro-CT）能够对这些聚合物支架的显著特征进行高速3D表征。微CT扫描，然后对连续图像切片进行3D重建，可以确定孔隙率、孔径、孔互连性、支柱尺寸和总体3D微结构。在这项研究中，四个不同的微结构的聚合物样品通过精密挤出沉积自由成型制造，随后通过微CT分析进行了表征。使用台式微型CT扫描仪以约19.1微米的分辨率检查每个样品。对每个样品的核心区域进行了2D分析和3D重建。这些结果表明，定性和定量分析的聚合物支架是可能的，使用micro-CT和三维重建技术。(C)2004 Wiley Periodicals，Inc.
One of the dominant approaches to tissue engineering is the seeding of biodegradable, biocompatible polymer scaffolds with progenitor cells prior to 3D culture or implantation. The microarchitecture of these scaffolds has direct effects upon the ability of cells to attach, migrate, and differentiate. Microtomographic (micro-CT) scanners enable high-speed 3D characterization of the salient features of these polymer scaffolds. A micro-CT scan followed by 3D reconstruction of serial image sections can determine porosity, pore size, pore interconnectivity, strut size, and overall 3D microarchitecture. In this study, four polymer samples with different microarchitectures were manufactured through precision extrusion deposition free-form fabrication and subsequently characterized through micro-CT analysis. A desktop micro-CT scanner was used to examine each sample at approximately 19.1-micron resolution. 2D analyses and 3D reconstructions of core regions of each sample were performed. These results illustrate that qualitative and quantitative analysis of polymer scaffolds is possible using micro-CT and 3D reconstruction techniques. (C) 2004 Wiley Periodicals, Inc.