Whole-genome approaches for large-scale gene identification and expression analysis in mammalian preimplantation embryos
Whole-genome approaches for large-scale gene identification and expression analysis in mammalian preimplantation embryos
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DOI：
10.1071/rd04075
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发表时间：
2005-01-01
影响因子：
1.9
通讯作者：
Adjaye, J
中科院分区：
文献类型：
--
作者：
Adjaye, J
如果我们要通过应用辅助生殖来干预和消除或减少与生长、疾病和不育相关的异常，那么阐明、解开和理解在生殖发育过程中转录控制的分子基础是至关重要的。重要的是，这些研究应该增强我们对基本生殖生物学及其在再生医学和畜牧业生产中的应用的知识。阻碍这些领域进展的一个主要障碍是从植入前胚胎的微量RNA成功生成其分子画像的能力。本综述描述了经典胚胎学与分子生物学、高通量基因组学和系统生物学融合以解决哺乳动物早期发育相关问题的各种方法。
The elucidation, unravelling and understanding of the molecular basis of transcriptional control during preimplantion development is of utmost importance if we are to intervene and eliminate or reduce abnormalities associated with growth, disease and infertility by applying assisted reproduction. Importantly, these studies should enhance our knowledge of basic reproductive biology and its application to regenerative medicine and livestock production. A major obstacle impeding progress in these areas is the ability to successfully generate molecular portraits of preimplantation embryos from their minute amounts of RNA. The present review describes the various approaches whereby classical embryology fuses with molecular biology, high-throughput genomics and systems biology to address and solve questions related to early development in mammals.