Natural/synthetic porous scaffold designs and properties for fibro-cartilaginous tissue engineering
Natural/synthetic porous scaffold designs and properties for fibro-cartilaginous tissue engineering
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DOI：
10.1177/0883911511420149
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发表时间：
2011-09-01
影响因子：
1.7
通讯作者：
Ambrosio, L.
中科院分区：
文献类型：
--
作者：
Borzacchiello, A.;Gloria, A.;Ambrosio, L.
本研究的目的是结合天然聚合物和合成聚合物的优点，制备和表征工程纤维软骨组织的支架。以聚己内酯增强的糖胺聚糖和透明质酸（HYAFF11）为基础制备多孔三维复合支架。将支架的力学性能作为时间的函数进行评估，并与植入人软骨细胞（构建物）并体外培养6周的支架进行比较。复合支架的孔隙率为68%，大孔相互连接，平均孔径为200 μ m，平衡膨胀率为350%，具有典型的大分子凝胶的弹性行为。复合结构维持软骨细胞表型，并随着细胞合成的大分子沉积而降解。该支架具有与纤维软骨组织相似的力学性能和能量耗散能力。
The goal of this study was to produce and characterize the scaffolds by combining the advantages of both natural and synthetic polymers for engineering fibro-cartilaginous tissues. Porous three-dimensional composite scaffolds were produced based on glycosaminoglycans and hyaluronic acid (HYAFF11) reinforced with polycaprolactone. The mechanical properties of scaffolds were evaluated as a function of time and compared with those of scaffolds seeded with human chondrocytes (constructs) and cultured in vitro up to 6 weeks. The composite scaffolds had a porosity of 68% with interconnected macropores with average pore sizes of 200 mu m, an equilibrium swelling of 350%, and a predominant elastic behavior, typical of a macromolecular gel. The composite constructs maintained chondrocyte phenotype and degraded with the deposition of macromolecules synthesized by the cells. The scaffold presented mechanical properties and the ability to dissipate energy similar to the fibro-cartilaginous tissue.