Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers
Difference in the Breast Milk Proteome between Allergic and Non-Allergic Mothers
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DOI：
10.1371/journal.pone.0122234
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发表时间：
2015-03-23
期刊：
影响因子：
3.7
通讯作者：
Wijga, Alet H.
中科院分区：
文献类型：
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作者：
Hettinga, Kasper A.;Reina, Fabiola M.;Wijga, Alet H.
背景母乳喂养与降低过敏和哮喘的患病率有关。然而，关于这种关系的研究结果各不相同，这可能部分与母乳成分的差异有关。特别地，过敏母亲和非过敏母亲的母乳成分可能不同。可能涉及的重要成分是母乳蛋白质，因为已知这些蛋白质调节新生儿的免疫发育。因此，本研究的目的是探讨20名过敏和非过敏母亲母乳中蛋白质的差异。从这个比较的结果，然后可以用来产生假设蛋白质过敏在他们的后代。MethodsMilk样本过敏和非过敏的母亲从PIAMA项目，一个前瞻性的出生队列研究的发病率，危险因素，和预防哮喘和吸入性过敏。非靶向蛋白质组学技术，液相色谱和质谱的基础上，被用来比较母乳过敏和非过敏mothers.Results19蛋白质，共364蛋白在两组中确定，显着不同的浓度之间的母乳过敏和非过敏的母亲。蛋白酶抑制剂和载脂蛋白在过敏性母乳中的浓度远高于非过敏性母亲。这些蛋白质已被建议与过敏和asthma.ConclusionsThe非靶向牛奶蛋白质组学分析采用母乳成分和过敏之间的关系，为今后的研究提供了新的目标。
BackgroundBreastfeeding has been linked to a reduction in the prevalence of allergy and asthma. However, studies on this relationship vary in outcome, which may partly be related to differences in breast milk composition. In particular breast milk composition may differ between allergic and non-allergic mothers. Important components that may be involved are breast milk proteins, as these are known to regulate immune development in the newborn. The objective of this study was therefore to explore differences in the proteins of breast milk from 20 allergic and non-allergic mothers. The results from this comparison may then be used to generate hypotheses on proteins associated with allergy in their offspring.MethodsMilk samples from allergic and non-allergic mothers were obtained from the PIAMA project, a prospective birth cohort study on incidence, risk factors, and prevention of asthma and inhalant allergy. Non-targeted proteomics technology, based on liquid chromatography and mass spectrometry, was used to compare breast milk from allergic and non-allergic mothers.ResultsNineteen proteins, out of a total of 364 proteins identified in both groups, differed significantly in concentration between the breast milk of allergic and non-allergic mothers. Protease inhibitors and apolipoproteins were present in much higher concentrations in breast milk of allergic than non-allergic mothers. These proteins have been suggested to be linked to allergy and asthma.ConclusionsThe non-targeted milk proteomic analysis employed has provided new targets for future studies on the relation between breast milk composition and allergy.