Molecular identification of a human DNA repair gene following DNA-mediated gene transfer
Molecular identification of a human DNA repair gene following DNA-mediated gene transfer
复制标题
DNA 介导的基因转移后人类 DNA 修复基因的分子鉴定
作者：
J. S. Rubin;A. Joyner;A. Bernstein;G. Whitmore
Although it has long been evident that the response of eukary-otes to DNA damaging agents is determined by the effectiveness of a variety of DNA repair systems, there is little detailed knowledge of the nature of these systems or the genes which control them. In humans, a number of hereditary conditions, including xeroderma pigmentosum, ataxia telangiectasia and Fanconi's anaemia, exhibit increased sensitivity to a variety of DNA damaging agents and a predisposition to cancer, suggesting a defect in some aspect of DNA repair1. This report describes the identification of a human DNA repair gene following DNA-mediated gene transfer into Chinese hamster ovary (CHO) mutant cells2,3, that like xeroderma pigmentosum cells, are sensitive to a variety of DNA damaging agents and are defective in the initial incision step of DNA repair4. The resulting transformants exhibit normal resistance to DNA damaging agents and independent transformants demonstrate a common set of human DNA sequences associated with a human DNA repair gene. These observations provide the basis for the isolation and characterization of the human genes responsible for DNA repair.
