Glycopeptidolipid Genotype Correlates With the Severity of Mycobacterium abscessus Lung Disease
Glycopeptidolipid Genotype Correlates With the Severity of Mycobacterium abscessus Lung Disease
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糖肽脂基因型与脓肿分枝杆菌肺病的严重程度相关
DOI：
10.1093/infdis/jiz475
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发表时间：
2020-04-01
影响因子：
6.4
通讯作者：
Chu, Haiqing
中科院分区：
文献类型：
--
作者：
Li, Bing;Ye, Meiping;Chu, Haiqing
背景：脓肿分枝杆菌的光滑型和粗糙型菌落形态与毒力相关，但一些分离株会形成光滑型和粗糙型两种菌落，这阻碍了成功的形态型鉴定。据报道，光滑/粗糙形态型也与糖肽脂（GPL）基因型有关。然而，GPL基因分型用于区分形态型的准确性以及GPL基因型与脓肿分枝杆菌肺病临床特征之间的关系尚未得到验证。
方法：对182例脓肿分枝杆菌肺病患者的菌落形态、GPL基因型和临床数据进行回顾性分析。
结果：在194株临床分离株中，分别有126株（65.0%）、15株（7.7%）和53株（27.3%）呈现粗糙型、光滑型和混合型菌落形态。糖肽脂基因分型表明，86.7%（15株中的13株）的光滑型分离株属于GPL - 野生型（WT）组，而98.4%（126株中的124株）的粗糙型分离株属于GPL - 突变型（MUT）组。因此，GPL基因分型可准确区分光滑型和粗糙型形态。混合型菌落形态也分为GPL - WT（18.9%）和GPL - MUT（81.1%）组。进一步分析显示，感染GPL - MUT组的患者基线临床特征明显更差，且肺病发作加重。
结论：糖肽脂基因分型可准确区分光滑型和粗糙型菌落形态。感染GPL - MUT基因型的患者临床特征更差，且肺病加重的风险更高。
Background. Smooth and rough colony morphotypes of Mycobacterium abscessus are associated with virulence, but some isolates form both smooth and rough colonies, impeding successful morphotype identification. Reportedly, smooth/rough morphotypes are also related to the glycopeptidolipid (GPL) genotype. However, the accuracy of GPL genotyping to discriminate morphotypes and the relationship between GPL genotype and clinical characteristics of M abscessus lung disease have not been verified.Methods. A retrospective analysis of colony morphology, GPL genotype, and clinical data from 182 patients with M abscessus lung disease was conducted.Results. Of 194 clinical isolates, 126 (65.0%), 15 (7.7%), and 53 (27.3%) exhibited rough, smooth, and mixed colony morphotypes, respectively. Glycopeptidolipid genotyping indicated that 86.7% (13 of 15) of smooth isolates belonged to the GPL-wild type (WT) group, whereas 98.4% (124 of 126) of rough isolates belonged to the GPL-mutant type (MUT) group. Therefore, GPL genotyping accurately distinguished between smooth and rough morphotypes. Mixed colony morphotypes were also divided into GPL-WT (18.9%) and GPL-MUT (81.1%) groups. Further analysis revealed that patients infected with the GPL-MUT group presented with significantly worse baseline clinical characteristics and exacerbated episodes of lung disease.Conclusions. Glycopeptidolipid genotyping accurately distinguishes smooth and rough colony morphotypes. Patients infected with the GPL-MUT genotype exhibit worse clinical characteristics and are at a higher risk of exacerbated lung disease.