Sample holder for small-angle x-ray scattering static and flow cell measurements
Sample holder for small-angle x-ray scattering static and flow cell measurements
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DOI：
10.1063/1.2194484
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发表时间：
2006-04-01
影响因子：
1.6
通讯作者：
Doniach, S
中科院分区：
文献类型：
--
作者：
Lipfert, J;Millett, IS;Doniach, S
我们提出了用于小角度 X 射线散射 (SAXS) 的样品架的设计，该样品架可用于静态测量和流动池测量，允许在这两种类型的测量之间切换，而无需重新对齐探测器和相机几何结构。该设备可以使用小至 16 μl 的样品量进行高信噪比实验，并且可以使用标准循环水浴进行温度控制。该装置已成功用于一系列生物 SAXS 测量，包括肽、去垢剂胶束、膜蛋白和核酸。作为性能测试，我们提供了在高级光子源的 BESSRC CAT 光束线 12-ID 处收集的马心细胞色素 c 的散射数据。设计图纸在补充材料中提供。 (c) 2006 年美国物理研究所。
We present the design of a sample holder for small-angle x-ray scattering (SAXS) that can be used for both static and flow cell measurements, allowing to switch between these two types of measurement without having to realign the detector and camera geometry. The device makes possible high signal-to-noise experiments with sample volumes as small as 16 mu l and can be thermocontrolled using a standard circulating water bath. The setup has been used successfully for a range of biological SAXS measurements, including peptides, detergent micelles, membrane proteins, and nucleic acids. As a performance test, we present scattering data for horse heart cytochrome c, collected at the BESSRC CAT beam line 12-ID of the Advanced Photon Source. The design drawings are provided in the supplementary material. (c) 2006 American Institute of Physics.