Recent fragmentation of the endangered Blakiston's fish owl (Bubo blakistoni) population on Hokkaido Island, Northern Japan, Revealed by Mitochondrial DNA and Microsatellite Analyses
Recent fragmentation of the endangered Blakiston's fish owl (Bubo blakistoni) population on Hokkaido Island, Northern Japan, Revealed by Mitochondrial DNA and Microsatellite Analyses
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DOI：
10.1186/s40851-015-0014-3
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发表时间：
2015-04-29
影响因子：
2.7
通讯作者：
Masuda, Ryuichi
中科院分区：
文献类型：
--
作者：
Omote, Keita;Nishida, Chizuko;Masuda, Ryuichi
简介：布氏鱼鸮（Bubo blakistoni）以前在日本北海道岛广泛分布，但现在只分布在有限的森林地区。世纪，由于猫头鹰栖息地的减少和破碎，北海道的种群数量减少。为了阐明种群结构和遗传多样性的时空变化，我们分析了439个个体在过去100 years.Results：我们发现了一个种群瓶颈和片段化事件表明线粒体DNA（mtDNA）单倍型和微卫星分析。有效种群规模的最低值出现在1980年代，这是根据微卫星数据通过矩和时间方法估计的。在mtDNA控制区发现了5种单倍型;大多数单倍型以前在北海道广泛分布，但在瓶颈期后在不同地区固定下来。mtDNA和微卫星数据表明，当地人群之间的遗传分化，可能会出现通过人口fragmentation.Conclusions：猫头鹰人口可能已被划分为有限的地区，由于通过人类活动的栖息地损失，并已失去了遗传变异，通过近亲繁殖的当地人群。我们的线粒体DNA和微卫星数据显示，遗传多样性下降，在当地人口，表明个人之间的重要性，保护该物种的北海道地区移动。
Introduction: Blakiston's fish owl (Bubo blakistoni) was previously widespread on Hokkaido Island, Japan, but is now distributed only in limited forest areas. The population size on Hokkaido decreased during the 20th century due to reduction and fragmentation of the owl's habitat. To elucidate temporal and spatial changes in population structure and genetic diversity, we analyzed 439 individuals collected over the last 100 years.Results: We detected a population bottleneck and fragmentation event indicated by mitochondrial DNA (mtDNA) haplotype and microsatellite analyses. The lowest value for effective population size, which was estimated by moment and temporal methods from microsatellite data, occurred in the 1980s. Five haplotypes were found in the mtDNA control region; most haplotypes were previously widespread across Hokkaido, but have become fixed in separate areas after the bottleneck period. Genetic differentiation among local populations, as indicated by both mtDNA and microsatellite data, likely arose through population fragmentation.Conclusions: The owl population may have been divided into limited areas due to loss of habitats via human activities, and have lost genetic variability within the local populations through inbreeding. Our mtDNA and microsatellite data show that genetic diversity decreased in local populations, indicating the importance of individuals moving between areas for conservation of this species on Hokkaido.