Decline in Presumptively Protective Gut Bacterial Species and Metabolites Are Paradoxically Associated with Disease Improvement in Pediatric Crohn's Disease During Enteral Nutrition
Decline in Presumptively Protective Gut Bacterial Species and Metabolites Are Paradoxically Associated with Disease Improvement in Pediatric Crohn's Disease During Enteral Nutrition
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DOI：
10.1097/mib.0000000000000023
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发表时间：
2014-05-01
影响因子：
4.9
通讯作者：
Edwards, Christine A.
中科院分区：
文献类型：
--
作者：
Gerasimidis, Konstantinos;Bertz, Martin;Edwards, Christine A.
背景：肠道微生物群与克罗恩病（CD）的发病机制有关。单纯肠内营养（EEN）是一种成功的治疗方法，但其作用机制尚不清楚。本研究评估了EEN期间粪便微生物群环境的一系列变化。研究方法：从CD儿童中采集5份粪便样本：EEN期间（开始、15、30、结束EEN约60天）采集4份，习惯性饮食采集第5份。从健康对照受试者中采集两份样本。粪便pH值，细菌代谢产物，全球微生物多样性丰度，组成的稳定性，和数量变化的总的和7个主要的细菌组先前牵连在CD。结果：总的来说，68个样本来自15个CD儿童和40个来自21个对照组。EEN期间粪便pH值、总硫化物含量升高，丁酸含量降低（均P < 0.05）。总体细菌多样性丰度下降（P < 0.05）;在EEN期间，对照组的微生物群组成稳定性高于CD儿童（P <0.05）。
Background: The gut microbiota is implicated in the pathogenesis of Crohn's disease (CD). Exclusive enteral nutrition (EEN) is a successful treatment, but its mode of action remains unknown. This study assessed serial changes in the fecal microbiota milieu during EEN. Methods: Five fecal samples were collected from CD children: 4 during EEN (start, 15, 30, end EEN approximately 60 days) and the fifth on habitual diet. Two samples were collected from healthy control subjects. Fecal pH, bacterial metabolites, global microbial diversity abundance, composition stability, and quantitative changes of total and 7 major bacterial groups previously implicated in CD were measured. Results: Overall, 68 samples were from 15 CD children and 40 from 21 control subjects. Fecal pH and total sulfide increased and butyric acid decreased during EEN (all P < 0.05). Global bacterial diversity abundance decreased (P < 0.05); a higher degree of microbiota composition stability was seen in control subjects than in CD children during EEN (at P