Vesicular stomatitis virus nucleocapsids diffuse through cytoplasm by hopping from trap to trap in random directions.
Vesicular stomatitis virus nucleocapsids diffuse through cytoplasm by hopping from trap to trap in random directions.
复制标题
水疱性口炎病毒核衣壳通过随机方向从一个陷阱跳跃到另一个陷阱而在细胞质中扩散。
DOI：
10.1038/s41598-020-66942-6
复制
发表时间：
2020
影响因子：
4.6
通讯作者：
Lyles,DouglasS
中科院分区：
文献类型：
--
作者：
Holzwarth,George;Bhandari,Arnav;Tommervik,Lucas;Macosko,JedC;Ornelles,DavidA;Lyles,DouglasS
在水泡性口炎病毒（VSV）感染后2-6小时内，新组装的VSV颗粒从感染细胞的表面释放。在这段时间里，病毒核糖核蛋白（RNP）颗粒（核衣壳）从细胞核附近的初始合成位点移动到细胞边缘，距离为5-10 μm。RNP颗粒的流体动力学半径（86 nm）排除了通过细胞骨架纤维网的简单扩散。为了揭示不同转运机制的相对重要性，通过荧光视频显微镜以100帧/秒的速度在感染后3至4小时内记录GFP标记的RNP颗粒在活A549细胞中的运动。贝叶斯模式识别软件对200多个RNP粒子轨迹的分析发现，3%的粒子显示出约1 μm/s的快速定向运动。97%的RNP颗粒在高斯宽度σ = 0.06 μm的小的近似圆形区域内抖动。这种“陷阱”内的运动是没有方向的。颗粒在阱中停留约1 s，然后跳到相邻的阱，其中心位移约0.17 μm。由于跳跃发生的频率比定向运动，RNP粒子的整体运输占主导地位的跳跃在这些实验的时间间隔。
Within 2–6 hours after infection by vesicular stomatitis virus (VSV), newly assembled VSV particles are released from the surface of infected cells. In that time, viral ribonucleoprotein (RNP) particles (nucleocapsids) travel from their initial sites of synthesis near the nucleus to the edge of the cell, a distance of 5–10 μm. The hydrodynamic radius of RNP particles (86 nm) precludes simple diffusion through the mesh of cytoskeletal fibers. To reveal the relative importance of different transport mechanisms, movement of GFP-labeled RNP particles in live A549 cells was recorded within 3 to 4 h postinfection at 100 frames/s by fluorescence video microscopy. Analysis of more than 200 RNP particle tracks by Bayesian pattern recognition software found that 3% of particles showed rapid, directional motion at about 1 μm/s, as previously reported. 97% of the RNP particles jiggled within a small, approximately circular area with Gaussian width σ = 0.06 μm. Motion within such “traps” was not directional. Particles stayed in traps for approximately 1 s, then hopped to adjacent traps whose centers were displaced by approximately 0.17 μm. Because hopping occurred much more frequently than directional motion, overall transport of RNP particles was dominated by hopping over the time interval of these experiments.