Cholera-toxin-enhanced growth of human breast cancer cell lines in vitro and in vivo: interaction with estrogen.
Cholera-toxin-enhanced growth of human breast cancer cell lines in vitro and in vivo: interaction with estrogen.
复制标题
霍乱毒素增强人乳腺癌细胞系的体外和体内生长：与雌激素的相互作用。
DOI：
10.1002/ijc.2910360411
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发表时间：
1985
影响因子：
6.4
通讯作者：
Welsch,CW
中科院分区：
文献类型：
--
作者：
Sheffield,LG;Welsch,CW
Cholera toxin (which increases intracellular cAMP levels) significantly (p< 0.05) increased the growth of MCF‐7, T47‐D and Hs578T human breast carcinoma cellsin vitro. The effect of cholera toxin on growth of MCF‐7 and T47‐D cells was more pronounced in the presence of 17β‐estradiol (p<0.05), indicating a synergism between cAMP and estradiol in growth control of estrogen‐receptor‐positive breast carcinoma cells. This interaction was not observed in the estrogenreceptor‐negative cell line Hs578T. Daily injections of cholera toxin into female athymic nude mice bearing MCF‐7 or Hs578T tumors resulted in significantly (p< 0.05) increased growth of the tumors. Cholera toxin treatments, in addition, significantly (p< 0.05) increased cAMP levels in tumor cells and tumor tissue,in vitroandin vivo, respectively. The results of this study clearly demonstrated that an increase in cAMP levels via cholera toxin treatment causes enhanced growth (in vitroandin vivo) of estrogen‐receptorpositive and ‐negative human breast carcinoma cells and, although estrogen alone was not mitogenic to the estrogen‐receptor‐positive breast carcinoma cellsin vitro, the steroid was mitogenic to these cells in the presence of elevated cellular cAMP levels.
