CD44 and associated markers in oral rinses and tissues from oral and oropharyngeal cancer patients
CD44 and associated markers in oral rinses and tissues from oral and oropharyngeal cancer patients
复制标题
DOI：
10.1016/j.oraloncology.2020.104720
复制
发表时间：
2020-07-01
期刊：
影响因子：
4.8
通讯作者：
Franzmann, Elizabeth J.
中科院分区：
文献类型：
--
作者：
Cohen, Erin R.;Reis, Isildinha M.;Franzmann, Elizabeth J.
目的：口腔和口咽鳞状细胞癌（OOPSCC）是一种使人衰弱的疾病。唾液冲洗液含有可溶性肿瘤标志物，包括CD 44（solCD 44）和总蛋白（TP），可以帮助检测和预后这些侵袭性肿瘤。在这里，我们的目的是检查这些唾液的生物标志物和组织标志物p16和CD 44之间的关系，并确定这些标志物是否可以预测无进展生存期（PFS）和总生存期（OS）。材料和方法：前瞻性研究，以更新生物标志物使用口腔冲洗液和组织之间的2007年和2012年在一个学术三级转诊中心的OOPSC患者。结果：82.8%为男性，84.4%为吸烟者，70.3%为T3-T4期，57.8%为淋巴结转移。19例（25%）p16阳性。在膜和细胞质中具有强组织CD 44表达的组具有比其他组（5.47 ng/ml）更高的solCD 44水平（平均10.73 ng/ml）（p = 0.033）。在p16通用大体肿瘤组织染色受试者的口腔冲洗液中，TP水平显著降低（平均值为0.80 vs 1.08 mg/ml; p = 0.039）。在多变量分析中，通用CD 44大体组织染色和TP水平>= 1 mg/ml表明PFS较差，后者也影响OS。患者生存与可溶性CD 44>= 5.33 ng/ml和TP >= 1 mg/ml相关。高solCD 44水平与强膜和胞质CD 44表达之间存在直接关联，高TP水平和外周/混合p16大体染色之间的差异。泊洛沙姆PFS和OS与口腔冲洗液中较高水平的solCD 44和蛋白质显著相关。
Objectives: Oral and oropharyngeal squamous cell carcinoma (OOPSCC) is a debilitating disease. Salivary rinses contain soluble tumor markers including CD44 (solCD44) and total protein (TP) that may aid detection and prognosis of these aggressive tumors. Here we aim to examine the relationship between these salivary biomarkers and tissue markers p16 and CD44 and determine whether these markers can predict progression-free survival (PFS) and overall survival (OS).Materials and methods: Prospective study to update biomarkers using oral rinses and tissues from OOPSC patients enrolled between 2007 and 2012 at an academic tertiary referral center. 64 cases from a 300-subject case-control study with archived tissue for immunohistochemistry were included.Results: 82.8% were male, 84.4% were ever smokers, 70.3% had disease stage T3-T4, and 57.8% presented with nodal disease. Nineteen patients (25%) were p16 positive. The group with strong tissue CD44 expression in membrane and cytoplasm had higher levels of solCD44 (mean 10.73 ng/ml) than other groups (5.47 ng/ml) (p = 0.033). TP levels were significantly reduced in oral rinses from subjects with p16 universal gross tumor tissue staining (mean 0.80 vs. 1.08 mg/ml; p = 0.039). On multivariate analysis, universal CD44 gross tissue staining and TP levels >= 1 mg/ml demonstrated poorer PFS, with the latter also affecting OS. Poorer survival was associated with soluble CD44 >= 5.33 ng/ml and TP >= 1 mg/ml.Conclusions: Direct associations were found between high solCD44 levels and strong membrane and cytoplasmic CD44 expression, and between high TP levels and peripheral/mixed p16 gross staining. Poorer PFS and OS are significantly associated with higher levels of solCD44 and protein in oral rinses.