Interface interaction between high-siliceous/calcareous mineral granules and model cell membranes dominated by electrostatic force
Interface interaction between high-siliceous/calcareous mineral granules and model cell membranes dominated by electrostatic force
复制标题
高硅质/钙质矿物颗粒与模型细胞膜之间静电力主导的界面相互作用
DOI：
10.1007/s11356-021-12584-8
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发表时间：
2021-01-28
影响因子：
5.8
通讯作者：
Zhang, Wei
中科院分区：
文献类型：
--
作者：
Chen, Mulan;Dong, Faqin;Zhang, Wei
高硅/钙质矿物颗粒可能通过附着于细胞膜而引起细胞毒性。在这项研究中，巨大的（GUV）和小单层囊泡（SUV）被用作模型膜研究高硅/钙质矿物颗粒（微米方解石，微米石英，纳米碳酸钙，纳米二氧化硅）和人工膜之间的相互作用。共聚焦激光扫描显微镜（CLSM）和荧光标记实验表明，纳米碳酸钙（纳米CaCO 3）和纳米二氧化硅（纳米SiO2）通过破坏带相反电荷的膜诱导凝胶化，表明静电力的重要作用。因此，矿物颗粒尺寸影响静电相互作用，从而导致膜的损坏。红外光谱和分子动力学研究表明，矿物颗粒主要与磷脂中的-PO 2 −、-OH和-C-N（CH 3）3+基团发生相互作用。纳米矿物和磷脂之间的静电力是更大的情况下SiO2相比，碳酸钙。此外，纳米SiO2与-PO 2 −基团形成最强的氢键，这一点通过FTIR证实。因此，纳米SiO2对膜的损伤最大。这项研究提供了一个更深入的了解机制，可吸入矿物颗粒和细胞膜之间的相互作用。
High-siliceous/calcareous mineral granules may cause cytotoxicity by attaching to cell membranes. In this research, giant (GUVs) and small unilamellar vesicles (SUVs) were used as model membranes for studying the interaction between high-siliceous/calcareous mineral granules (micro calcite, micro quartz, nano calcium carbonate, and nano silica) and artificial membranes. Confocal laser scanning microscopy (CLSM) and fluorescence labeling experiments suggest that nano calcium carbonate (nano CaCO3) and nano silica (nano SiO2) induce gelation by disrupting the oppositely charged membranes, indicating the important role of electrostatic forces. Thereby, the mineral granule size affects the electrostatic interactions and thus leading to the damage of the membranes. FTIR spectra and molecular dynamics reveal that mineral granules mainly interact with -PO2−, -OH, and -C-N(CH3)3+groups in phospholipids. The electrostatic force between nano minerals and phospholipids is greater in the case SiO2when compared to CaCO3. Moreover, nano SiO2forms the strongest hydrogen bond with the -PO2−group as confirmed by FTIR. Thus, nano SiO2causes the greatest damage to membranes. This research provides a deeper understanding of the mechanism regarding the interaction between inhalable mineral granules and cell membranes.