Postnatal food restriction in the rat as a model for a low nephron endowment
Postnatal food restriction in the rat as a model for a low nephron endowment
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DOI：
10.1152/ajprenal.00158.2006
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发表时间：
2006-11-01
影响因子：
4.2
通讯作者：
Delemarre-van de Waal, Henriette A.
中科院分区：
文献类型：
--
作者：
Schreuder, Michiel F.;Nyengaard, Jens R.;Delemarre-van de Waal, Henriette A.
低肾单位禀赋可能与高血压有关。肾发生是导致人类妊娠36周之前形成肾单位的过程，并且可能受到许多因素如宫内生长受限和早产的抑制。为了研究肾小球数量少的后果，已经开发了动物模型。我们描述了一个模型，出生后的食物限制在大鼠中，其中胎仔数增加到20只幼崽，这导致生长受限。在大鼠中，活跃的肾发生持续到出生后第8天，这与我们模型中的生长限制相吻合。设计为基础的体视学方法被用来估计肾小球数量和体积。我们的研究结果显示，与对照组大鼠（39，600个肾小球/肾）相比，出生后限食大鼠的肾小球数量（30，800个肾小球/肾）减少了25%，P < 0.001。生长受限大鼠的平均肾小球体积增加了35%（P = 0.006）。肾小球体积与肾小球数目呈显著负相关（r =-0.76，P < 0.001）。我们的结论是，大鼠出生后的食物限制导致低肾单位禀赋与代偿性扩大。因此，这是一个合适的模型，研究宫内生长受限或早产对肾脏发育的影响和肾小球数量减少的后果，在以后的生活。
A low nephron endowment may be associated with hypertension. Nephrogenesis is the process that leads to the formation of nephrons until week 36 of gestation in humans and may be inhibited by many factors like intrauterine growth restriction and premature birth. To study the consequences of a low glomerular number, animal models have been developed. We describe a model of postnatal food restriction in the rat in which litter size is increased to 20 pups, which leads to growth restriction. In the rat, active nephrogenesis continues until postnatal day 8, which coincides with the growth restriction in our model. Design-based stereological methods were used to estimate glomerular number and volume. Our results show an similar to 25% lower glomerular number in rats after postnatal food restriction (30,800 glomeruli/kidney) compared with control rats (39,600 glomeruli/kidney, P < 0.001). Mean glomerular volume was increased by 35% in the growth-restricted rats (P = 0.006). There was a significant negative correlation between glomerular volume and glomerular number (r = -0.76, P < 0.001). We conclude that postnatal food restriction in the rat leads to a low nephron endowment with compensatory enlargement. It is therefore a suitable model to study the effect of intrauterine growth restriction or prematurity on kidney development and the consequences of a reduced glomerular number in later life.