ENDOTHELIN-1 STIMULATES HUMAN MONOCYTES IN-VITRO TO RELEASE TNF-ALPHA, IL-1-BETA AND IL-6
ENDOTHELIN-1 STIMULATES HUMAN MONOCYTES IN-VITRO TO RELEASE TNF-ALPHA, IL-1-BETA AND IL-6
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DOI：
10.1155/s0962935193000596
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发表时间：
1993-12-01
影响因子：
4.6
通讯作者：
KONOPSKI, ZS
中科院分区：
文献类型：
--
作者：
HELSET, E;SILDNES, T;KONOPSKI, ZS
炎症性疾病时血浆和组织中内皮素-1(ET1)水平升高，提示ET-1在炎症反应的病理生理学中发挥作用。作者研究了ET-1对单核细胞和单核细胞来源的巨噬细胞释放细胞因子的影响。ET-1以剂量和时间依赖的方式促进TNF-α、IL-1β和IL-6的分泌。最适ET-1浓度范围为0.01~1 nM。最大的反应是细胞因子释放增加了200%到400%。时程研究表明，ET-1诱导的细胞因子在单核细胞和巨噬细胞中的模式不同，尽管在单核细胞和巨噬细胞上清液中观察到早期的肿瘤坏死因子-α的升高。综上所述，ET-1刺激单核细胞和巨噬细胞释放细胞因子，从而显示ET-1在调节炎症反应中的潜在作用。
INCREASED plasma- and tissue levels of endothelin-1 (ET1) during inflammatory diseases, have suggested a role of ET-1 in the pathophysiotogy of inflammatory reactions. The authors have studied the effect of ET-1 on cytokine release from monocytes and monocyte-derived macrophages. ET-1 increased secretion of TNF-alpha, IL-1beta and IL-6 in a dose- and time-dependent manner. Optimal ET-1 concentration ranged from 0.01 to 1 nM. The maximal response was a 200 to 400% increase in cytokine release. A time-course study revealed that the pattern of cytokines induced by ET-1 was different in monocytes and macrophages, although an early increase in TNF-alpha was observed in both monocyte and macrophage supernatants. In conclusion, ET-1 stimulates monocytes and macrophages to release cytokines thereby demonstrating a potential role for ET-1 in regulation of inflammatory responses.